Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
A total of eight membrane-associated tyrosine protein kinases have been identified within the Src gene family. These include c-Src, c-Yes, Fyn, Lck, Hck, Lyn, Blk and c-Fgr. The major translational product of the human Lck gene is a lymphocyte-specific tyrosine kinase designated pp56Lck. This is a membrane-associated molecule, most likely via covalently associated myristate at the amino terminus. The Lck gene has been shown to undergo rearrangement and overexpression in some murine lymphomas. In human studies, it has been demonstrated that the Lck gene is localized to a site in the genome which undergoes frequent chromosomal abnormalities in lymphomas and neuroblastomas. A novel Lck signaling intermediate, designated Lck BP-1, associates directly with the Lck SH3 domian via two proline-rich regions. Lck BP-1 also contains four tandem 37 amino acid repeats that form a putative helix-loop-helix DNA binding motif. Immunoprecipitation studies have shown that Lck BP-1 will co-immunoprecipitate with Lck from T cell lysates. Lck BP-1 is tyrosine phosphorylated in T cells subsequent to TCR activation.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
What is the recommended fixation method for immunofluorescence staining with Lck BP-1 (9): sc-135972 monoclonal antibody?
Richiesta da: jerojero
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here:
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