Anticorpo LAMP-2 (M3/84) raccomandato per il rilevamento di LAMP-2 di origine mouse, rat e human in WB, IP, IF, IHC(P) e FCM

Anticorpo LAMP-2 (M3/84): sc-19991

Anticorpo LAMP-2 (M3/84) valutazione 4.1 di 5 di 8.
  • y_2021, m_6, d_15, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.17
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_8
  • loc_it_IT, sid_19991, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 135ms
  • REVIEWS, PRODUCT
loading
5
4
4
1
3
3
2
0
1
0

Schede tecniche

    • LAMP-2 Antibody M3/84 è un monoclonale di ratto IgG1, citato in 49 pubblicazioni, fornito in 200 µg/ml
    • epitope mapping to immunoadsorbent purified mouse macrophage glycoprotein fraction
    • LAMP-2 Antibody (M3/84) é raccomandato per il rilevamento di LAMP-2 di origine mouse, rat e human in WB, IP, IF, IHC(P) e FCM
    • LAMP-2 Antibody (M3/84) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • disponibile coniugato a Alexa Fluor® 405 per IF, IHC(P) e FCM
    • Contattaci per ricevere un FREE 10 µg sample di LAMP-2 (M3/84): sc-19991.
loading

Can LAMP-2 (M3/84): sc-19991 be used to IHQ (not paraffin-embedded) from frozen tissue sections?

Richiesta da: yarasi
Thank you for your question. Yes, this antbody is recommended and warrantied for IF and for IHC on fresh frozen tissue sections.
Risposta da: Tech Service
Data di pubblicazione: 2019-12-09

Hallo What secondary antibodies can be used with this product?

Richiesta da: Soll
Thank you for your question. You can use any secondary antibody that is specific against rat IgG1 antibodies to detect LAMP-2 Antibody (M3/84): sc-19991. For further information please contact our technical service directly.
Risposta da: SCBT Heidelberg Support
Data di pubblicazione: 2019-01-06

Can LAMP-2 (M3/84): sc-19991 be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Richiesta da: jenni
Thank you for your question. Yes, LAMP-2 (M3/84): sc-19991 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Risposta da: Technical Support 15
Data di pubblicazione: 2017-02-07
  • y_2021, m_6, d_15, h_18CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.17
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_3
  • loc_it_IT, sid_19991, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 163ms
  • QUESTIONS, PRODUCT
Rated 3 di 5 di da Lots of noisy background with this antibody for Lots of noisy background with this antibody for murine cells.
Data di pubblicazione: 2019-12-04
Rated 5 di 5 di da wb 5%milk blocked, diluted in 1:500, the band is clear,and the backgroud is clean
Data di pubblicazione: 2017-12-13
Rated 5 di 5 di da Lots of signal There was a strong band at 120 KDa in mouse aorta and NIH/3T3 samples by WB
Data di pubblicazione: 2017-08-11
Rated 5 di 5 di da Very popular for IF/IHC(P)! This rat monoclonal antibody, clone (M3/84) is a very popular choice for IF and IHC(P) applications for detection of LAMP-2 in mouse, rat and human samples.
Data di pubblicazione: 2017-01-24
Rated 4 di 5 di da Used in WB Used in WB, worked fine.
Data di pubblicazione: 2015-07-30
Rated 3 di 5 di da Large smear observed by Western Blot using J774A Large smear observed by Western Blot using J774A.1 whole cell lysate. -SCBT QC
Data di pubblicazione: 2015-04-04
Rated 5 di 5 di da Two publications cite the use of this antibody Two publications cite the use of this antibody with mouse aorta and NIH/3T3 samples, analyzed by Western Blot. -SCBT Publication Review
Data di pubblicazione: 2015-03-27
Rated 3 di 5 di da Large bulbous bands (indicative of overloaded Large bulbous bands (indicative of overloaded gel) observed by Western Blot using MCP-5, NIH/3T3 and RAW 264.7 whole cell lysates. -SCBT QC
Data di pubblicazione: 2014-10-02
  • y_2021, m_6, d_15, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.17
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_8
  • loc_it_IT, sid_19991, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 14ms
  • REVIEWS, PRODUCT