Anticorpo LAMP-2 (H4B4) raccomandato per il rilevamento di LAMP-2 di origine human in WB, IP, IF, IHC(P) e FCM

Anticorpo LAMP-2 (H4B4): sc-18822

Anticorpo LAMP-2 (H4B4) valutazione 4.6 di 5 di 37.
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Schede tecniche

    • Anti-LAMP-2 Antibody H4B4 è un monoclonale di topo IgG1 κ LAMP-2 antibody, citato in 276 pubblicazioni, fornito in 200 µg/ml
    • raised against adherent spleen cells of human origin
    • LAMP-2 Antibody (H4B4) é raccomandato per il rilevamento di LAMP-2 di origine human in WB, IP, IF, IHC(P) e FCM
    • LAMP-2 Antibody (H4B4) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • disponibile coniugato a Alexa Fluor® 405 per IF, IHC(P) e FCM
    • Contattaci per ricevere un FREE 10 µg sample di LAMP-2 (H4B4): sc-18822.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for LAMP-2 Antibody (H4B4) for WB and IHC(P) applications. These reagents are now offered in bundles with LAMP-2 Antibody (H4B4) (see ordering information below).
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The LAMP2A bands from MDA-MB-468 (human triple negative breast cancer cells) and HeLa cells are not lined up. They are both human cells, but why the size of LAMP2A are different?

Richiesta da: Irene63
Thank you for your question. We do not currently have any published Western blot data in MDA-MB-468 whole cell lysate, so it is unclear what your question is referring to specifically. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Risposta da: Technical Support
Data di pubblicazione: 2019-08-17

What would be suitable of blocking and incubations for this antibody between milk and BSA..? Thanks in advance

Richiesta da: phani
Thank you for your question. We recommend using 5% nonfat milk + TBST (available pre-made as our TBS Blotto A: sc-2333) for blocking and both primary and secondary incubations with this LAMP-2 Antibody (H4B4): sc-18822. A link to our recommended protocol for Western blotting is provided below as a reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Risposta da: Technical Support
Data di pubblicazione: 2017-09-01

What is the recommended fixative for this antibody in immunofluorescence?

Richiesta da: Imagenation
Thank you for your question. We recommend using methanol as a fixative for immunofluorescent staining of cultured cells with this mouse monoclonal, LAMP-2 Antibody (H4B4): sc-18822. A link to our complete recommended protocol is provided below. https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Risposta da: Technical Support
Data di pubblicazione: 2017-04-13

What is the recommended secondary reagent to be used with this mouse monoclonal primary by immunofluorescence?

Richiesta da: jenni
We recommend using one our exclusive Mouse IgG Binding Proteins conjugated to PE, FITC or a CruzFluor fluorescent dye as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Risposta da: Santa Cruz TS
Data di pubblicazione: 2016-12-14

Does LAMP-2 Antibody (H4B4): sc-18822 detect the LAMP-2 of equine origin?

Richiesta da: jenni
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Risposta da: Santa Cruz TS
Data di pubblicazione: 2016-12-14
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Rated 4 di 5 di da IF LCLs L'anticorpo è stato utilizzato per IF su linee cellulari linfoblastoidi e abbiamo ottenuto un buon segnale!
Data di pubblicazione: 2021-04-30
Rated 5 di 5 di da Great features I bought this 1 month ago, and this antibody works very well in my IF assay.
Data di pubblicazione: 2021-01-21
Rated 5 di 5 di da Works in IF As expected for clone H4B4, consistently good staining of endogenous protein in cell lines (1:100, FA-fixed). Also working in Western.
Data di pubblicazione: 2020-12-04
Rated 4 di 5 di da buon anticorpo testato su 3 linee di tumore mammario umano ERBB2+, da segnale forte e specifico per immunofluorescenza (1:200-1:400). Non testato per WB.
Data di pubblicazione: 2020-10-05
Rated 5 di 5 di da Excellent in Western Blotting Western Blotting of HepG2 cell lysate Dilution 1:1000 Specific and strong signal at the expected molecular weight
Data di pubblicazione: 2020-03-02
Rated 5 di 5 di da good antibody works pretty good in my hands, I would highly recommend this antibody.
Data di pubblicazione: 2018-04-05
Rated 2 di 5 di da Did not perform as expected Used in IHC on kidney samples. Cryosectioned kidney slides were blocked in BSA/NDS 5% for an hour, washed in PBS, then incubated in 1:200 dilution overnight in 4C. Secondary was applied for an hour, then washed, mounted and imaged. The samples' imaging show only nonspecific staining, and chromagen development shows nothing useful. I had hoped for more clearly labelled LAMP-2. We will be trying another antibody for our IHC work.
Data di pubblicazione: 2018-01-29
Rated 5 di 5 di da WB THE ANTIBODY IS DILUTED IN 1:500,AND THE BAND IS CLEAN AND IN THE RIGHT PLACE.
Data di pubblicazione: 2017-12-12
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