Anticorpo LAMP-2 (ABL-93) raccomandato per il rilevamento di LAMP-2 di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA

Anticorpo LAMP-2 (ABL-93): sc-20004

Anticorpo LAMP-2 (ABL-93) valutazione 4.5 di 5 di 15.
  • y_2021, m_6, d_15, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.17
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_15
  • loc_it_IT, sid_20004, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 134ms
  • REVIEWS, PRODUCT
loading
5
12
4
0
3
2
2
0
1
1

Schede tecniche

    • LAMP-2 Antibody ABL-93 è un monoclonale di ratto IgG2a, citato in 30 pubblicazioni, fornito in 200 µg/ml
    • raised against glycoprotein fractions purified from BALB/c mouse embryo 3T3 cell line
    • LAMP-2 Antibody (ABL-93) é raccomandato per il rilevamento di LAMP-2 di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA
    • LAMP-2 Antibody (ABL-93) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • disponibile coniugato a Alexa Fluor® 405 per IF, IHC(P) e FCM
    • Contattaci per ricevere un FREE 10 µg sample di LAMP-2 (ABL-93): sc-20004.
loading

Can LAMP-2 (ABL-93): sc-20004 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Richiesta da: jenniferc
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, LAMP-2 (ABL-93) is available in a variety of direct conjugations, such as HRP, PE, FITC, Alexa Fluor® 405, AlexaFluor 488 and AlexaFluor 647.
Risposta da: Technical Support 6
Data di pubblicazione: 2021-02-09
  • y_2021, m_6, d_15, h_18CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.17
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_it_IT, sid_20004, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 118ms
  • QUESTIONS, PRODUCT
Rated 5 di 5 di da wb use it to text the autophasy signal in the primary neuron cell of rats,and it worked
Data di pubblicazione: 2017-12-13
Rated 3 di 5 di da I don't think this is a right pattern for LAMP2 I got the sample sack two weeks ago. Antibody for detecting mouse specifical LAMP2 is not easy to look for. Although I got the same signal as in the product description, I don't think it is a right pattern for LAMP2.
Data di pubblicazione: 2017-09-19
Rated 3 di 5 di da can detect signal but not effecience can detect signal but not effecience。 the signal is enough strong
Data di pubblicazione: 2017-09-05
Rated 1 di 5 di da Did not work for brain lysate Suppose to be a negative control in brain lysate in western blot, but band did not show up
Data di pubblicazione: 2017-08-10
Rated 5 di 5 di da works ok for IHC\ DC 2.4 cells (murine) cells. It however worked with the 2' AB specifically that can recognize IgG2b form. It did not work well in my hand with IgG (H+L) 2' Abs
Data di pubblicazione: 2017-07-16
Rated 5 di 5 di da good result of WB I use this antibody detect mosue brain tissue expression the target protein.we recommend transferred the protein onto PVDF membranes with a semi-dry transfer method,and check and confirm the proteinis transfer to the membrance successfully,then incubate the antibody with the membrance for 1hour at RT ,the expose time shoudnot be too long,it may cause dark background.
Data di pubblicazione: 2017-07-01
Rated 5 di 5 di da Clear band This antibody works well for WB, diluted with 1/600, shows good quality
Data di pubblicazione: 2017-05-10
Rated 5 di 5 di da WB is better Works perfectly and great for Western Blot, when dilution 1:500 most obvious effect.
Data di pubblicazione: 2017-04-11
  • y_2021, m_6, d_15, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.17
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_15
  • loc_it_IT, sid_20004, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 15ms
  • REVIEWS, PRODUCT