Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
KHS CRISPR/Cas9 KO Plasmid (h): sc-405817
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KHS CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the KHS genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: KHS Antibody (E-5): sc-374070
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KHS CRISPR/Cas9 KO Plasmid (h) | sc-405817 | 20 µg | $397.00 |
Overview
Mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), also known as kinase homologous to SPS1/STE20 (KHS), is a Ste20 family serine/threonine kinase that functions upstream of MAPK signaling modules. MAP4K5 contributes to stress-responsive and inflammatory signaling through regulation of JNK and related pathways, influencing transcriptional programs that control proliferation, apoptosis, and cytoskeletal dynamics. In human cells, KHS activity has been linked to modulation of innate immune responses and metabolic signaling, positioning MAP4K5 within broader networks that integrate receptor-mediated cues with kinase cascades. Dysregulated MAP4K5 signaling has been investigated in contexts such as oncogenic pathway rewiring and inflammatory disease mechanisms, supporting its use as a node for pathway dissection.
KHS CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MAP4K5 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MAP4K5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MAP4K5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish KHS protein expression.
This CRISPR knockout system enables efficient generation of MAP4K5-deficient cell models for investigation of KHS signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MAP4K5 exon(s) critical for KHS function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MAP4K5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by KHS CRISPR/Cas9 KO Plasmid (h) and KHS CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MAP4K5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by KHS HDR Plasmid (h) and KHS HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MAP4K5 homology arms to support homology-directed repair at defined MAP4K5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.