Anticorpo JNK1/3 (F-5) raccomandato per il rilevamento di all JNK1 and JNK3 p46 and p54 isoforms di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA

Anticorpo JNK1/3 (F-5): sc-514539

Anticorpo JNK1/3 (F-5) valutazione 3.5 di 5 di 2.
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Schede tecniche

    • Anti-JNK1/3 Antibody F-5 è un monoclonale di topo IgG3 κ JNK1/3 antibody, citato in 1 pubblicazioni, fornito in 200 µg/ml
    • specifico per un epitopo mappato tra gli amminoacidi 364-385 vicino al C-terminus di JNK1 p46 di origine human
    • raccomandato per il rilevamento di all JNK1 and JNK3 p46 and p54 isoforms di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA
    • Vedere JNK (D-2): sc-7345 for JNK1/3 per anticorpi coniugati, tra cui AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 e 790.
    • Contattaci per ricevere un FREE 10 µg sample di JNK1/3 (F-5): sc-514539.
    • m-IgG3 BP-HRP is the preferred secondary detection reagent for JNK1/3 Antibody (F-5) for WB and IHC(P) applications. This reagent is now offered in a bundle with JNK1/3 Antibody (F-5) (see ordering information below).
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What application is the blocking peptide sc-514539 P appropriate for?

Richiesta da: Trav11
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Risposta da: Technical Support
Data di pubblicazione: 2017-02-27
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Rated 3 di 5 di da Not very good I had tested it in C42B and VCaP cell lines. I got a lot of bands and I don't know which one it is.
Data di pubblicazione: 2017-10-11
Rated 4 di 5 di da worked In A549 cells 46kD JNK1 was detected. 54 kD was not. The detected band was decreased by siJNK1 transfection. There are no non-specific band. 1/1000 dil in 2% BSA TBST, o/n reaction.
Data di pubblicazione: 2017-03-24
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