Integrin alpha 4 Antibody C-2 è un monoclonale di topo IgG1 κ, citato in 1 pubblicazioni, fornito in 200 µg/ml
generato contro gli amminoacidi 796-1005 mappati vicino al C-terminus di Integrin α4 di origine human
Integrin alpha 4 Antibody (C-2) é raccomandato per il rilevamento di Integrin α4 di origine human in WB, IP, IF e ELISA
Integrin alpha 4 Antibody (C-2) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
Contattaci per ricevere un FREE 10 µg sample di Integrin α4 (C-2): sc-365569.
m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgG1 BP-HRP (mouse IgG1 binding protein-HRP) are the preferred secondary detection reagents for Integrin α4 Antibody (C-2) for WB applications. These reagents are now offered in bundles with Integrin α4 Antibody (C-2) (see ordering information below).
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Labware ships FedEx Ground free of charge to the contiguous US
Integrins are heterodimers composed of noncovalently associated transmembrane α and β subunits. The 16 α and 8 β subunits heterodimerize to produce more than 20 different receptors. Most integrin receptors bind ligands that are components of the extracellular matrix, including fibronectin, collagen and vitronectin. Certain integrins can also bind to soluble ligands such as fibrinogen, or to counterreceptors on adjacent cells such as the intracellular adhesion molecules (ICAMs), leading to aggregation of cells. Ligands serve to cross-link or cluster integrins by binding to adjacent integrin receptors; both receptor clustering and ligand occupancy are necessary for the activation of integrin-mediated responses. In addition to mediating cell adhesion and cytoskeletal organization, integrins function as signaling receptors. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What blocking solution should I use with Integrin α4 (C-2): sc-365569 monoclonal antibody for Western blot?
Richiesta da: Trav11
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here:
Rated 1 di
Didnot work for WBdid not work for western - samples were human cells in culture
Data di pubblicazione: 2017-11-21
Rated 5 di
good featurewe used this antibody to detect the expression of integrin in glioma cell lysate by western blot
Data di pubblicazione: 2017-09-25
Rated 5 di
Works very wellI used this antibody to detect ITGA4 in mouse bone marrow derived macrophages, and it picks up a simple right band!
Data di pubblicazione: 2017-04-26
Rated 5 di
Steven Pelech at Kinexus da
Highly potent and fairly specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was very strong detection of the ~150 kDa target protein in a HeLa/Jurkat cell lysate mix, but the target was not detected in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested or in the HeLa/Jurkat cell lysate mix, except for a medium-strong immunoreactive 36 kDa protein found in both mice and humans.
Data di pubblicazione: 2016-11-10
Rated 5 di
Western blot analysis shows Integrin 4 expressionWestern blot analysis shows Integrin 4 expression in Jurkat (A) and MOLT-4 (B) whole cell lysates. -SCBT QC
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn