Anticorpo insulin R beta (CT-3) raccomandato per il rilevamento di insulin Rβ di origine mouse, rat e human in WB, IP, IF e IHC(P)

Anticorpo insulin Rβ (CT-3): sc-57342

Anticorpo insulin Rβ (CT-3) valutazione 4.3 di 5 di 18.
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Schede tecniche

    • Anti-insulin R beta Antibody CT-3 è un monoclonale di topo IgG1 (kappa light chain) insulin Rβ antibody fornito in 200 µg/ml
    • generato contro il C-terminus di insulin receptor di origine human
    • insulin R beta Antibody (CT-3) é raccomandato per il rilevamento di insulin Rβ di origine mouse, rat e human in WB, IP, IF e IHC(P); non cross-reattivo con insulin-like growth factor (IGF)receptors
    • insulin R beta Antibody (CT-3) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • Vedi m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), le nostre alternative ricombinanti ai reagenti secondari convenzionali anti-mouse IgG altamente raccomandate.
    • Contattaci per ricevere un FREE 10 µg sample di insulin Rβ (CT-3): sc-57342.
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Is a blocking pepide available for the sc-57342 Insulin receptor antibody?

Richiesta da: PaulDA
Thank you for your question. There is no blocking peptide available for this specific antibody.
Risposta da: Technical Support
Data di pubblicazione: 2019-09-11

Can you provide information about the hybridoma cell line this antibody was produced from?

Richiesta da: KMitok
Thank you for your question. The specific clone name for this monoclonal antibody is CT-3. If you require additional information, please contact our Technical Service Department for assistance.
Risposta da: Technical Support
Data di pubblicazione: 2019-09-11

I have a clear band of approximately 45 KDa with this antibody. Do you know what it can be? Thank you so much!

Richiesta da: Jimena
Thank you for your question. We do not have any information regarding a 45 kDa sized band when using this antibody. Please contact our Technical Support Department for help with troubleshooting the antibody, or more detailed information about the expected molecular weight. Please call us toll-free at (800) 457-3801 option 2, or use the live chat on our website.
Risposta da: Technical Support
Data di pubblicazione: 2018-11-22

In the data sheet , it was written the antigen is C-terminal , does the C-terminal region contain Tryosine domain?

Richiesta da: TANG QIAN
Thank you for your question. This antibody was made against a recombinant protein fragment including the C-terminal 100 amino acid residues of human insulin receptor (accession#P06213). There are tyrosine domains within the C-terminus but since the antibody has not been epitope mapped, I do not know if the exact epitope includes a Tyrosine. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Risposta da: Tech Service
Data di pubblicazione: 2017-09-05

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with insulin Rβ (CT-3): sc-57342 monoclonal antibody?

Richiesta da: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Risposta da: Technical Support 4
Data di pubblicazione: 2017-01-10
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Rated 4 di 5 di da Application of insulin Rβ Antibody for IHC and WB The insulin Rβ Antibody (CT-3) SC-57342 is suitable for intracellular localization studies of the insulin receptor in 3T3-L1 adipocytes at different stages of differentiation by immunofluorescence and confocal microscopy . The antibody can be also used for Western blot studies of protein extracts from 3T3-L1 adipocytes, including quantitative analyses.
Data di pubblicazione: 2018-09-13
Rated 5 di 5 di da Works great! Works at 1:50 dilution for immunofluorescence in hES cells
Data di pubblicazione: 2018-03-01
Rated 5 di 5 di da Works great for IHC in frozen mouse liver sections I used this at a 1:250 dilution on frozen mouse liver sections and it worked great! Did not work well in paraffin embedded sections.
Data di pubblicazione: 2018-01-03
Rated 4 di 5 di da nice band the antibody was tested in western blot on rat tissue samples and worked well
Data di pubblicazione: 2017-12-13
Rated 4 di 5 di da positive result in WB worked well for detection of insulin receptor from cell lysate
Data di pubblicazione: 2017-09-06
Rated 3 di 5 di da ISR b This antibody was used for Western blotting of MCF7 and T47D cell lysate。It did not work in T47D。It had some signal with a little background in MCF7.
Data di pubblicazione: 2017-06-15
Rated 4 di 5 di da relative high sensitivity show some cross reaction with IGF-1Rb, but generally ok to detect IRb
Data di pubblicazione: 2017-05-17
Rated 1 di 5 di da Confronto tra anticorpo poli e monoclonale A parità di proteine caricate (40ug) e condizioni di diluizione anticorpo (1:1000 in milk 2.5%) il nuovo anticorpo monoclonale (sc-57342) non funziona in modo soddisfacente rispetto al vecchio anticorpo policlonale (sc-711).
Data di pubblicazione: 2017-04-03
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