Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IL-15Rα Double Nickase Plasmid (h): sc-416909-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-15Rα Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IL-15Rα Double Nickase Plasmid (h) and IL-15Rα Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IL15RA. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IL-15Rα Antibody (G-3): sc-374023
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-15Rα Double Nickase Plasmid (h) | sc-416909-NIC | 20 µg | $410.00 | |||
IL-15Rα Double Nickase Plasmid (h2) | sc-416909-NIC-2 | 20 µg | $410.00 |
Human IL15RA encodes the interleukin-15 receptor alpha chain (IL-15Rα), a high-affinity binding component that captures IL-15 and presents it in trans to IL-2/IL-15Rβ and common γ chain–expressing cells. This receptor complex activates JAK1/JAK3-dependent signaling with downstream STAT5, PI3K–AKT, and MAPK pathway engagement, shaping lymphocyte survival, proliferation, and effector differentiation. IL-15Rα is central to natural killer cell and memory CD8+ T cell homeostasis and influences cytokine network dynamics within inflamed tissues. Dysregulated IL15RA expression or signaling has been linked to immune-mediated pathology and altered immune surveillance in inflammatory and oncologic settings, making it a relevant target for mechanistic studies of cytokine-driven immunity.
IL-15Rα Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IL15RA locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IL15RA. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IL15RA function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IL15RA-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.