Anticorpo IKK alpha (C-6) raccomandato per il rilevamento di IKKα di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA

Anticorpo IKKα (C-6): sc-166231

Anticorpo IKKα (C-6) valutazione 5.0 di 5 di 3.
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Schede tecniche

    • IKK alpha Antibody C-6 è un monoclonale di topo IgG1 κ, citato in 6 pubblicazioni, fornito in 200 µg/ml
    • raised against amino acids 248-452 mapping at the C-terminus of IKKα of human origin
    • raccomandato per il rilevamento di IKKα di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA
    • Vedere IKKα (B-8): sc-7606 for IKKα per anticorpi coniugati, tra cui AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 e 790.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for IKKα Antibody (C-6) for WB and IHC(P) applications. These reagents are now offered in bundles with IKKα Antibody (C-6) (see ordering information below).
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I am using Ikka (C-6): sc-166231 to stain fixed frozen sections. Do you recommend antigen retrieval?

Richiesta da: signalingJoan
Thank you for your question. Yes, we recommend antigen retrieval for this antibody. You can find the recommended protocol, including antigen retrieval, here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Risposta da: Tech Service
Data di pubblicazione: 2019-03-26

I am using IKKα (C-6): sc-166231 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Richiesta da: Germaine
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Risposta da: Technical Support
Data di pubblicazione: 2017-02-24
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Rated 5 di 5 di da Produced excellent immunofluorescence cytoplasmic Produced excellent immunofluorescence cytoplasmic staining in methanol-fixed NIH/3T3 cells. -SCBT QC
Data di pubblicazione: 2015-05-16
Rated 5 di 5 di da Publication in WB with mouse renal tissue Publication in WB with mouse renal tissue. -SCBT QC
Data di pubblicazione: 2015-04-11
Rated 5 di 5 di da Works great for Western in Jurkat whole cell Works great for Western in Jurkat whole cell lysate. -SCBT QC
Data di pubblicazione: 2015-03-03
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