IFN-γ/Interferon gamma Antibody (DB-10): sc-59990
- IFN-γ/Interferon gamma Antibody (DB-10) is a mouse monoclonal IgG1 provided at 100 µg/ml
- produced by eight stable hybridoma cell lines
- recommended for detection of IFN-γ of mouse and rat origin by WB and IP
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for IFN-γ/Interferon gamma Antibody (DB-10). This work is in progress.
IFN-γ Antibody (DB-10) is a mouse monoclonal IgG1 antibody that detects interferon gamma (IFN-γ) in mouse and rat samples through applications such as western blotting (WB) and immunoprecipitation (IP). Anti-IFN-γ antibody (DB-10) plays a crucial role in studying immune response, as IFN-γ is primarily produced by activated CD4+/CD8+ lymphocytes and natural killer cells in response to antigens, mitogens, or alloantigens. IFN-γ monoclonal antibody (DB-10) helps researchers examine this cytokine′s essential role in modulating T cell growth and differentiation while inhibiting B cell proliferation, thereby influencing the overall immune landscape. IFN-γ′s active form is a homodimer, with each subunit comprising six helices, and this dimeric structure is stabilized by non-covalent interactions at the interface of the helices, which is vital for biological activity. The precursor of IFN-γ consists of 166 amino acids, including a 23 amino acid secretory sequence, and functionality can be affected by various post-translational modifications, including glycosylation, which leads to the existence of multiple forms under non-denaturing conditions, such as dimers and tetramers. IFN-γ′s ability to interact with specific receptors and other proteins in the immune system underscores its importance in antiviral and antiparasitic defense mechanisms, making IFN-γ (DB-10) antibody an invaluable tool for researchers studying immune responses and cytokine signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
IFN-γ/Interferon gamma Antibody (DB-10) References:
- Transcription of the interferon gamma (IFN-gamma)-inducible chemokine Mig in IFN-gamma-deficient mice. | Mahalingam, S., et al. 2001. J Biol Chem. 276: 7568-74. PMID: 11024052
- Sensitization of IFN-gamma Jak-STAT signaling during macrophage activation. | Hu, X., et al. 2002. Nat Immunol. 3: 859-66. PMID: 12172544
- Annexin V associates with the IFN-gamma receptor and regulates IFN-gamma signaling. | Leon, C., et al. 2006. J Immunol. 176: 5934-42. PMID: 16670301
- Tryptophan depletion and the kinase GCN2 mediate IFN-γ-induced autophagy. | Fougeray, S., et al. 2012. J Immunol. 189: 2954-64. PMID: 22896630
- Inflammasome-dependent IFN-γ drives pathogenesis in Streptococcus pneumoniae meningitis. | Mitchell, AJ., et al. 2012. J Immunol. 189: 4970-80. PMID: 23071286
- CD160 is essential for NK-mediated IFN-γ production. | Tu, TC., et al. 2015. J Exp Med. 212: 415-29. PMID: 25711213
- Ubiquilin 1 Promotes IFN-γ-Induced Xenophagy of Mycobacterium tuberculosis. | Sakowski, ET., et al. 2015. PLoS Pathog. 11: e1005076. PMID: 26225865
- IFN-γ, CXCL16, uPAR: potential biomarkers for systemic lupus erythematosus. | Wen, S., et al. 2018. Clin Exp Rheumatol. 36: 36-43. PMID: 28628472
- IFN-γ and CD25 drive distinct pathologic features during hemophagocytic lymphohistiocytosis. | Humblet-Baron, S., et al. 2019. J Allergy Clin Immunol. 143: 2215-2226.e7. PMID: 30578871
- IL-11 prevents IFN-γ-induced hepatocyte death through selective downregulation of IFN-γ/STAT1 signaling and ROS scavenging. | Miyawaki, A., et al. 2019. PLoS One. 14: e0211123. PMID: 30779746
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IFN-γ/Interferon gamma Antibody (DB-10) | sc-59990 | 100 µg/ml | $322.00 |