H2-I/A Antibody (NIMR-4): sc-52548
- H2-I/A Antibody (NIMR-4) is a rat monoclonal IgG2b, cited in 1 publications, provided at 200 µg/ml
- raised against B-lymphocytes of mouse origin
- H2-I/A Antibody (NIMR-4) is recommended for detection of non-polymorphic I-A-encoded epitope on MHC class II antigens of mouse origin by IF and FCM
- Anti-H2-I/A Antibody (NIMR-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for H2-I/A Antibody (NIMR-4). This work is in progress.
H2-I/A Antibody (NIMR-4) is a rat monoclonal IgG2b antibody that detects the non-polymorphic I-A-encoded epitope on MHC class II antigens of mouse origin by immunofluorescence (IF) and flow cytometry (FCM). Anti-H2-I/A antibody (NIMR-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Major histocompatibility complex (MHC) class II molecules, which include the target of H2-I/A (NIMR-4) monoclonal antibody, play a crucial role in the immune response by presenting processed exogenous antigens to helper T lymphocytes. These molecules are primarily located on the surface of antigen-presenting cells, such as B cells and dendritic cells, where they facilitate the activation of T cells. The ability of MHC class II molecules to bind and present longer peptides, typically ranging from 13 to 18 amino acids, is essential for the effective recognition of a diverse array of antigens, thereby influencing the adaptive immune response. The interaction between MHC class II molecules and T cell receptors is vital for the initiation of immune responses, making the study of these proteins and their interactions critical for understanding immune system function and developing immunotherapies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
H2-I/A Antibody (NIMR-4) References:
- Functional and phenotypic evidence for presentation of E alpha 52-68 structurally related self-peptide(s) in I-E alpha-deficient mice. | Viret, C. and Janeway, CA. 2000. J Immunol. 164: 4627-34. PMID: 10779766
- Characterization of murine dendritic cells derived from adherent blood mononuclear cells in vitro. | Agger, R., et al. 2000. Scand J Immunol. 52: 138-47. PMID: 10931381
- Polymorphism and evolution of HLA class I and II genes and molecules. | Little, AM. and Parham, P. 1999. Rev Immunogenet. 1: 105-23. PMID: 11256568
- Accessory proteins that control the assembly of MHC molecules with peptides. | Van Kaer, L. 2001. Immunol Res. 23: 205-14. PMID: 11444385
- The major histocompatibility complex of the rat (Rattus norvegicus). | Günther, E. and Walter, L. 2001. Immunogenetics. 53: 520-42. PMID: 11685465
- Molecular genetics of the HLA complex. | Fischer, GF. and Mayr, WR. 2001. Wien Klin Wochenschr. 113: 814-24. PMID: 11732117
- Enhancement of MHC class II-restricted responses by receptor-mediated uptake of peptide antigens. | Zaliauskiene, L., et al. 2002. J Immunol. 169: 2337-45. PMID: 12193699
- On the complexity of self. | Rudensky AYu, ., et al. 1991. Nature. 353: 660-2. PMID: 1656278
- A novel MHC class II epitope expressed in thymic medulla but not cortex. | Murphy, DB., et al. 1989. Nature. 338: 765-8. PMID: 2469959
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
H2-I/A Antibody (NIMR-4) | sc-52548 | 200 µg/ml | $322.00 | |||
H2-I/A Antibody (NIMR-4) AC | sc-52548 AC | 500 µg/ml, 25% agarose | $424.00 | |||
H2-I/A Antibody (NIMR-4) HRP | sc-52548 HRP | 200 µg/ml | $322.00 | |||
H2-I/A Antibody (NIMR-4) FITC | sc-52548 FITC | 200 µg/ml | $336.00 | |||
H2-I/A Antibody (NIMR-4) PE | sc-52548 PE | 200 µg/ml | $349.00 | |||
H2-I/A Antibody (NIMR-4) Alexa Fluor® 488 | sc-52548 AF488 | 200 µg/ml | $364.00 | |||
H2-I/A Antibody (NIMR-4) Alexa Fluor® 546 | sc-52548 AF546 | 200 µg/ml | $364.00 | |||
H2-I/A Antibody (NIMR-4) Alexa Fluor® 594 | sc-52548 AF594 | 200 µg/ml | $364.00 | |||
H2-I/A Antibody (NIMR-4) Alexa Fluor® 647 | sc-52548 AF647 | 200 µg/ml | $364.00 | |||
H2-I/A Antibody (NIMR-4) Alexa Fluor® 680 | sc-52548 AF680 | 200 µg/ml | $364.00 | |||
H2-I/A Antibody (NIMR-4) Alexa Fluor® 790 | sc-52548 AF790 | 200 µg/ml | $364.00 |