GMF-γ Double Nickase Plasmid (m): sc-425658-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GMF-γ Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GMF-γ Double Nickase Plasmid (m) and GMF-γ Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Gmfg. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GMF-γ Double Nickase Plasmid (m) | sc-425658-NIC | 20 µg | $410.00 | |||
GMF-γ Double Nickase Plasmid (m2) | sc-425658-NIC-2 | 20 µg | $410.00 |
Gmfg encodes glia maturation factor gamma (GMF-γ), an actin-regulatory protein enriched in hematopoietic and immune lineages that modulates Arp2/3-dependent actin branching and debranching to shape lamellipodia dynamics. Through control of cytoskeletal remodeling, GMF-γ influences chemotaxis, adhesion, and phagocytic behaviors, linking it to signaling nodes downstream of integrins and small Rho-family GTPases that coordinate cell migration. Altered GMF-γ activity can perturb leukocyte trafficking and inflammatory responses, making Gmfg a useful locus for studying immune cell motility and microenvironmental interactions in mouse models. These processes are relevant to mechanistic investigations of inflammation-associated tissue remodeling and tumor–immune crosstalk without implying therapeutic outcomes.
GMF-γ Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Gmfg locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Gmfg. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Gmfg function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Gmfg-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.