Anticorpo GFAP (F-7) raccomandato per il rilevamento di GFAP di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA

Anticorpo GFAP (F-7): sc-166458

Anticorpo GFAP (F-7) valutazione 5.0 di 5 di 5.
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Schede tecniche

    • GFAP Antibody F-7 è un monoclonale di topo IgG1 κ, citato in 17 pubblicazioni, fornito in 200 µg/ml
    • specifico per un epitopo mappato tra gli amminoacidi 2-19 al N-terminus di GFAP di origine human
    • GFAP Antibody (F-7) é raccomandato per il rilevamento di GFAP di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA
    • GFAP Antibody (F-7) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • Contattaci per ricevere un FREE 10 µg sample di GFAP (F-7): sc-166458.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GFAP Antibody (F-7) for WB and IHC(P) applications. These reagents are now offered in bundles with GFAP Antibody (F-7) (see ordering information below).
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I am using GFAP Antibody (F-7): sc-166458 for Western blot. How do I know if the band I am detecting is specific?

Richiesta da: DefinitelyNotMatt
Thank you for your question. We recommend using the blocking peptide sc-166458 P as a negative control. Antibody binding to antigen may be blocked/competed by pre-absorption with the blocking peptide. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization Please contact Technical Service if you have further questions concerning this product.
Risposta da: Technical Support 5
Data di pubblicazione: 2017-01-24
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Rated 5 di 5 di da Excellent choice for IHC and WB GFAP Antibody (F-7) is a very good antibody for IHC(P) and WB. Also very popular choice for IF as indicated in the numerous recent product citations.
Data di pubblicazione: 2017-01-07
Rated 5 di 5 di da Nice immunofluorescence staining on rat striatum Nice immunofluorescence staining on rat striatum sections. -SCBT Publication Review
Data di pubblicazione: 2015-04-18
Rated 5 di 5 di da Worked well for WB using synaptoneurosomes Worked well for WB using synaptoneurosomes. -SCBT Publication Review
Data di pubblicazione: 2015-02-22
Rated 5 di 5 di da Published using F Published using F-7 for IF on rat glial extract. -SCBT Publication Review
Data di pubblicazione: 2015-01-03
Rated 5 di 5 di da Strong clean band in human GFAP transfected Strong clean band in human GFAP transfected 293T whole cell lysates. -SCBT QC
Data di pubblicazione: 2013-11-18
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