Anticorpo GATA-3 (HG3-35) raccomandato per il rilevamento di GATA-3 di origine human in WB, IP, IF e IHC(P)

Anticorpo GATA-3 (HG3-35): sc-269

Anticorpo GATA-3 (HG3-35) valutazione 4.8 di 5 di 6.
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Schede tecniche

    • GATA-3 Antibody HG3-35 è un monoclonale di topo IgG1 κ, citato in 47 pubblicazioni, fornito in 200 µg/ml
    • generato contro gli amminoacidi 1-264 di GATA-3 di origine human
    • GATA-3 Antibody (HG3-35) é raccomandato per il rilevamento di GATA-3 di origine human in WB, IP, IF e IHC(P)
    • GATA-3 Antibody (HG3-35) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • reagente TransCruz per utilizzo in Gel Supershift e ChIP (sc-269 X, 200 µg/0.1 ml)
    • Contattaci per ricevere un FREE 10 µg sample di GATA-3 (HG3-35): sc-269.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for GATA-3 Antibody (HG3-35) for WB and IHC(P) applications. These reagents are now offered in bundles with GATA-3 Antibody (HG3-35) (see ordering information below).
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What is the difference between HG3-31 and HG3-35?

Richiesta da: Vincent YU
Thank you for your question. These are two different antibody clones directed to detect GATA-3. However, they do target different epitopes, and have different reactivities. For example, sc-268 (HG3-31) is mouse, rat and human reactive, while sc-269 (HG3-35) is human reactive only.
Risposta da: Tech Service
Data di pubblicazione: 2019-12-09

The GATA-3 antibody (HG3-31) works OK in MCF-7 cells, but it has high background in WB with JEG-3 cells. May I have free sample of HG3-35 to test with JEG-3 cells?

Richiesta da: monhu
Thank you for your question. Please contact Technical Service or your local distributor for information on how to receive a free 10 ug sample of this monoclonal antibody.
Risposta da: Tech Service
Data di pubblicazione: 2019-05-24

When should the TransCruz reagent sc-269 X be used instead of the standard form sc-269?

Richiesta da: cjMara
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Risposta da: Technical Support
Data di pubblicazione: 2017-02-24
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Rated 4 di 5 di da GATA-3 Works well in IHC -FFPE. very clean and intense staining observed.
Data di pubblicazione: 2018-04-20
Rated 5 di 5 di da Imunofluorescência Excelente! Pedi uma amostra grátis para testar em imunoflourescência e funcionou bem!
Data di pubblicazione: 2018-03-07
Rated 5 di 5 di da Works well in FFPE Great choice when working with paraffin-embedded sections in IHC. Antigen retrieval recommended.
Data di pubblicazione: 2017-01-24
Rated 5 di 5 di da Works well in WB with mouse spleen cells Works well in WB with mouse spleen cells -SCBT Publication Review
Data di pubblicazione: 2015-02-15
Rated 5 di 5 di da Good for ChIP with Jurkat cell lysates Good for ChIP with Jurkat cell lysates -SCBT Publication Review
Data di pubblicazione: 2015-02-02
Rated 5 di 5 di da Low background and strong bands in WB with MOLT Low background and strong bands in WB with MOLT-4 nuclear extract and CCRF-HSB-2 whole cell lysate. -SCBT QC
Data di pubblicazione: 2013-02-23
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