Anticorpo GATA-3 (HG3-31) raccomandato per il rilevamento di GATA-3 di origine mouse, rat, human e avian in WB, IP, IF e IHC(P)

Anticorpo GATA-3 (HG3-31): sc-268

Anticorpo GATA-3 (HG3-31) valutazione 4.1 di 5 di 23.
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Schede tecniche

    • Anti-GATA-3 Antibody HG3-31 è un monoclonale di topo IgG1 κ GATA-3 antibody, citato in 401 pubblicazioni, fornito in 200 µg/ml
    • generato contro il ricombinante GATA-3 di origine human
    • GATA-3 Antibody (HG3-31) é raccomandato per il rilevamento di GATA-3 di origine mouse, rat, human e avian in WB, IP, IF e IHC(P)
    • GATA-3 Antibody (HG3-31) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • reagente TransCruz per utilizzo in Gel Supershift e ChIP (sc-268 X, 200 µg/0.1 ml)
    • Contattaci per ricevere un FREE 10 µg sample di GATA-3 (HG3-31): sc-268.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GATA-3 Antibody (HG3-31) for WB and IHC(P) applications. These reagents are now offered in bundles with GATA-3 Antibody (HG3-31) (see ordering information below).
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We bought this antibody, GATA-3 Antibody (HG3-31): sc-268, and did IF on the sections ( 5 um) of mouse breast tumors after antigen unmasking (PH 6, pressure cooking 2 min). However, the location of GATA3 is in the cytoplasm, not in the nuclei. Why?

Richiesta da: Mmcc
Thank you for your question. Please contact our Technical Service Department for help with troubleshooting the antibody. Please call us toll-free at (800) 457-3801 option 2, or use the live chat on our website.
Risposta da: Technical Support
Data di pubblicazione: 2018-11-22

Does the HG3-31 antibody show significant cross-reactivity with the other GATA factors (that is GATA-1, GATA-2, GATA-4, GATA-5 or GATA-6)?

Richiesta da: wliam
Thank you for your question. This mouse monoclonal, GATA-3 Antibody (HG3-31): sc-268, is specific for GATA-3 and does not cross-react with other GATA factors. The antibody is extremely well characterized, and has been cited in the literature over 300 times. If you would be interested in testing a 10 µg sample of this antibody, for a limited time, we are offering a sample size. Please contact our Technical Service Department either by phone at (800) 457-3801 option 2, email [email protected], or use our live chat!
Risposta da: Technical Support
Data di pubblicazione: 2018-09-10

may i ask that this antibody can detect GATA3 in pig?

Richiesta da: leseichi
Thank you for your question. We have not tested our mouse monoclonal, GATA-3 Antibody (HG3-31): sc-268, in pig. However, the antibody is raised against a recombinant protein immunogen that shares 94% sequence identity with the published protein for GATA-3 in pig (NP_001038032). Based on this sequence identity, we would expect this antibody to cross-react with GATA-3 in pig. If you are interested in testing a free sample of this antibody, please contact our Technical Service Department. As part of a limited time promotion, we are offering a free 10µg sample of our mouse monoclonal, GATA-3 Antibody (HG3-31): sc-268. Please call our Asian Support office toll-free at 800-820-8626, email [email protected], or contact us by live chat.
Risposta da: Technical Support
Data di pubblicazione: 2017-02-01

Dilution and protocol to use on Dako Autostainer Link 48? Thanks

Richiesta da: joann
Thank you for your question. Our recommended immunohistochemistry protocol for using our monoclonal, GATA-3 Antibody (HG3-31): sc-268, is provided below. We have not used this antibody in combination with the Dako Autostainer Link 48 system, so we are unable to provide you any specific recommendations. However, this antibody has been validated for IHC on formalin-fixed paraffin-embedded tissue sections so it should be compatible. You may want to contact the other supplier to see if they can offer any specific recommendations for antibody usage with their equipment. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Risposta da: Technical Support
Data di pubblicazione: 2017-01-25

How reliable does the HG3-31 perform in mouse tissues?

Richiesta da: Professor Griffin
Thank you for your question. The HG3-31 clone is well established for mouse on mouse histological detection; PMID 19661437, PMID 17359917, PMID 17237387.
Risposta da: Technical Support
Data di pubblicazione: 2016-12-20
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Rated 1 di 5 di da Not working Cannot get a specific band and got multiple unspecific bands.
Data di pubblicazione: 2020-10-08
Rated 1 di 5 di da not working I purchased this ab two weeks ago since was one of the best rated for GATA3. I tried BSA and MILK blocking, 1 to 200 dilution and increasing concentrations of MCF7 cell lysates. It did not work so far
Data di pubblicazione: 2018-10-29
Rated 4 di 5 di da anti GATA3 sc-268 anti GATA3 sc-268 , dilution 1/500 in human breast cancer cells
Data di pubblicazione: 2018-10-19
Rated 4 di 5 di da Strong band Strong band seen in WB using GATA-3 in whole cell lysate.
Data di pubblicazione: 2017-10-10
Rated 5 di 5 di da GATA-3 I used the antibody for immunofluroscence staining and it worked excellent
Data di pubblicazione: 2017-09-22
Rated 4 di 5 di da GATA-3 wb is okay WB: 1:1000, mouse spleen tissue, 5% BSA block, antibody duluting with 5%BSA in TBST buffer. WB, two bind attached photos
Data di pubblicazione: 2017-09-02
Rated 4 di 5 di da Good antibody The antibody works great for westernblotting using mouse brain tissue at 1:500 dilution.
Data di pubblicazione: 2017-07-24
Rated 5 di 5 di da western blot I used it to do western blot .It have signal in Hela cell line at 1:500.
Data di pubblicazione: 2017-05-31
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