Anticorpo G alpha s (12) raccomandato per il rilevamento di Gα s di origine mouse, rat e human in WB e IP

Anticorpo Gα s (12): sc-135914

Anticorpo Gα s (12) valutazione 4.7 di 5 di 11.
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Schede tecniche

    • G alpha s Antibody 12 è un monoclonale di topo IgG1 fornito in 200 µg/ml
    • generato contro gli amminoacidi 11-21 di Gα s di origine human
    • raccomandato per il rilevamento di Gα s di origine mouse, rat e human in WB e IP
    • disponibile coniugato ad agarosio per IP; ad HRP per WB, IHC(P) ed ELISA
    • Vedi m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), le nostre alternative ricombinanti ai reagenti secondari convenzionali anti-mouse IgG altamente raccomandate.
    • Contattaci per ricevere un FREE 10 µg sample di Gα s (12): sc-135914.

Hi, Is this MAb specific for Gα-s? because the description of MAb for Gα-olf says, it can recognize both forms s and olf and in our study, we need to identify which one of the two is involved, so specificity is very necessary.

Richiesta da: ASM25
Thank you for your question. The antibody was raised against amino acids 11-21 of G s of human origin. The exact binding region of this antibody within the antigen has not been mapped. This antibody has not been tested experimentally for potential cross-reactivity under denaturing conditions in a Western Blot or native conditions in IP against different proteins. You might want to align the antigen sequence against the protein form of interest in order to determine potential reactivity. based on the antigen sequence we do not expect relevant cross-reactivity against G -olf. Please contact our Technical Service by phone or email or by Live Chat if you have any further questions.
Risposta da: SCBT Heidelberg Support
Data di pubblicazione: 2018-11-27

Hi, How can I have a 10ul trial sample of the Gα s Antibody (12): sc-135914 antibody. Thanks

Richiesta da: Zhong Ding
Thank you for your inquiry. For a limited time, we are offering a free 10 µg sample of this antibody. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website.
Risposta da: Technical Support 15
Data di pubblicazione: 2018-02-01

For Western Blot, is it recommended to use denatured or non-denatured conditions with this antibody?

Richiesta da: TinTin
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Risposta da: Technical Support 9
Data di pubblicazione: 2017-01-12

Can you recommend a positive control to use with Gα s (12): sc-135914 for Western blot?

Richiesta da: TinTin
We recommend Jurkat Whole Cell Lysate (sc-2204) or rat or mouse brain tissue extract (sc-2392 or sc-2253) as positive controls as we have Western blot data showing specific detection of Gα s in these samples.
Risposta da: Technical Support 9
Data di pubblicazione: 2017-01-12
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Rated 4 di 5 di da Strong Antibody Signal for WB We tried a sample antibody on WB, signal was strong, but we got two bands.
Data di pubblicazione: 2017-11-03
Rated 5 di 5 di da no problem The WB of the antibody was detected in the tissues of rat cerebellum, the signals were clear, specific and unique.
Data di pubblicazione: 2017-10-26
Rated 5 di 5 di da Worked good This antibody sc-135914 works very well in WB, TBS 5% milk, diluted 1/250.
Data di pubblicazione: 2017-08-25
Rated 5 di 5 di da works well It works well in western blotting at dilution of 1:500 analysis Human liver tissue .
Data di pubblicazione: 2017-02-25
Rated 3 di 5 di da Moderate specificity Galpha-S (12/sc-135914): Moderate specificity: detects multiple bands from HEK293 lysate, of which one is the correct MW for Galpha-S. Poor sensitivity - may be optimized.
Data di pubblicazione: 2017-02-09
Rated 5 di 5 di da Superb western Very nice western blot results; strong signal with low backgrond
Data di pubblicazione: 2017-01-31
Rated 5 di 5 di da Great for WB A proven reagent for WB detection. It provides great signal and clear specific bands. It is reactive with mouse, rat, and human and has been published with several times.
Data di pubblicazione: 2017-01-30
Rated 5 di 5 di da Various expression levels of the G s protein Various expression levels of the G s protein is demonstrated by Western Blot using Jurkat, HEK293, TT, K562 and MCF-7 whole cell lysates. -SCBT QC
Data di pubblicazione: 2015-04-12
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