Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Fucokinase CRISPR/Cas9 KO Plasmid (h): sc-409449
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Fucokinase CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Fucokinase genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Fucokinase Antibody (F-9): sc-377371
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Fucokinase CRISPR/Cas9 KO Plasmid (h) | sc-409449 | 20 µg | $397.00 |
Overview
Human FUK encodes fucokinase, a cytosolic kinase that phosphorylates L-fucose to fucose-1-phosphate in the fucose salvage pathway, supplying substrate for GDP-fucose biosynthesis. By contributing to cellular pools of activated fucose, FUK supports fucosylation of glycoproteins and glycolipids, a modification that modulates receptor signaling, cell–cell adhesion, and immune recognition. Altered fucose metabolism and fucosylation patterns have been linked to dysregulated inflammation, oncogenic signaling, and changes in tumor cell invasiveness, making FUK a relevant node for studying glycan-dependent phenotypes. Functional interrogation of FUK helps connect metabolic flux through nucleotide-sugar pathways to glycosyltransferase output and downstream changes in membrane and secreted glycoproteomes.
Fucokinase CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FUK gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FUK together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FUK open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Fucokinase protein expression.
This CRISPR knockout system enables efficient generation of FUK-deficient cell models for investigation of Fucokinase signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FUK exon(s) critical for Fucokinase function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FUK genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Fucokinase CRISPR/Cas9 KO Plasmid (h) and Fucokinase CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FUK locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Fucokinase HDR Plasmid (h) and Fucokinase HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FUK homology arms to support homology-directed repair at defined FUK target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.