FMNL3 CRISPR/Cas9 KO Plasmid (h): sc-406244
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FMNL3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FMNL3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FMNL3 CRISPR/Cas9 KO Plasmid (h) | sc-406244 | 20 µg | $397.00 |
Overview
FMNL3 (formin-like protein 3) is a Rho GTPase–regulated actin nucleation and elongation factor that coordinates remodeling of the cortical actin cytoskeleton. It supports formation of filopodia and lamellipodia, regulates cell polarity, and contributes to directed migration through pathways linked to CDC42/RAC signaling and cytoskeletal dynamics. FMNL3 activity influences membrane protrusion, adhesion turnover, and intracellular trafficking processes that depend on actin assembly. Dysregulated FMNL3 expression or signaling has been associated with phenotypes relevant to tumor cell invasion and metastasis in multiple model systems, making it a useful target for mechanistic studies of motility and cytoskeletal control.
FMNL3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FMNL3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FMNL3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FMNL3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FMNL3 protein expression.
This CRISPR knockout system enables efficient generation of FMNL3-deficient cell models for investigation of FMNL3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FMNL3 exon(s) critical for FMNL3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FMNL3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FMNL3 CRISPR/Cas9 KO Plasmid (h) and FMNL3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FMNL3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FMNL3 HDR Plasmid (h) and FMNL3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FMNL3 homology arms to support homology-directed repair at defined FMNL3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.