FLUO 3, sale di pentaammonio, è un chelante e indicatore di Ca2+ che opera a lunghezze d'onda di eccitazione maggiori. Ciò offre una minore interferenza da parte dell'autofluorescenza endogena, oltre alla possibilità di utilizzo simultaneo con altre sonde fluorescenti.
FLUO 3, Pentaammonium Salt (CAS 134907-84-9) Referenze
New calcium indicators and buffers with high selectivity against magnesium and protons: design, synthesis, and properties of prototype structures: R.Y. Tsien; Biochemistry 19, 2396 (1980) Fluorescence measurement and photochemical manipulation of cytosolic free calcium: R.Y. Tsien; TINS 11, 419 (1988) Kinetics of calcium binding to fluo-3 determined by stopped-flow fluorescence: M. Eberhard & P. Erne; BBRC 163, 309 (1989) Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores: A. Minta, et al.; J. Biol. Chem. 264, 8171 (1989) Photochemically generated cytosolic calcium pulses and their detection by fluo-3: J.P.Y. Kao, et al.; J. Biol. Chem. 264, 8179 (1989) Use of fluo-3 to measure cytosolic Ca2+ in platelets and neutrophils. Loading cells with the dye, calibration of traces, measurements in the presence of plasma, and buffering of cytosolic Ca2+: J.E. Merritt, et al.; Biochem. J. 269, 513 (1990) Improved method for measuring intracellular Ca++ with fluo-3: G.T. Rijkers, et al.; Cytometry 11, 923 (1990) Control of mitochondrial matrix calcium: studies using fluo-3 as a fluorescent calcium indicator: A. Saavedra-Molina, et al.; BBRC 167, 148 (1990) Effects of photolabile calcium chelators on fluorescent calcium indicators: R.S. Zucker; Cell Calcium 13, 29 (1992)