Ethidium bromide CAS: 1239-45-8
MF: C21H20N3•Br
MW: 394.3
An intercalating fluorescent dye reagent for DNA/RNA staining.

Ethidium bromide (CAS 1239-45-8)

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Ethidium bromide is rated 5.0 out of 5 by 1.
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Nomi alternativi: EtBr; Dromilac; 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide
Applicazione: Ethidium bromide is an intercalating fluorescent dye reagent for DNA/RNA staining
Numero CAS: 1239-45-8
Peso molecolare: 394.3
Formula molecolare: C21H20N3•Br
Informazioni supplementari: This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
Solo per uso in Ricerca. Non previsto per Uso Diagnostico o Terapeutico.
* Vedere Certificato di Analisi per informazioni sul lotto specifico (incluso il contenuto d'acqua).

Ethidium bromide is a polyaromatic fluorescent small molecule and nucleic acid intercalator widely used as a stain in DNA and RNA electrophoresis. Upon intercalation into the hydrophobic region between nucleic acid base pairs, fluorescence-quenching water molecules associated to the Ethidium bromide structure are displaced and the bound Ethidium bromide then fluoresces very strongly compared to free Ethidium bromide (~25 fold increase in dsDNA). Intercalation distorts the ribonucleic acid structure and impedes natural DNA interactions, presenting a powerful mutagen effect associated with Ethidium bromide. This imposition on DNA structure also makes Ethidium bromide an inhibitor of DNA polymerase.


Referenze

1. Elliott, W.H. 1963. Biochem. J. 86: 562-567. PMID: 16748992
2. Lepecq, J.B., et al. 1964. C.R. Hebd. Seances Acad. Sci. 259: 1786-1789. PMID: 14196160
3. LePecq, J.B. and Paoletti, C. 1967. J. Mol. Biol. 27: 87-106. PMID: 6033613
4. Douthart, R.J., et al. 1973. Biochemistry. 12: 214-220. PMID: 4630220
5. El Hamalawi, A.R., et al. 1975. Anal. Biochem. 67: 384-391. PMID: 1163763
6. Karsten, U. and Wollenberger, A. 1977. Anal. Biochem. 77: 464-470. PMID: 842829
7. Laine, B., et al. 1984. Biochem. Biophys. Res. Commun. 119: 1147-1153. PMID: 6370250
8. Moore, S.P. and Sutherland, B.M. 1985. Anal. Biochem. 144: 15-19. PMID: 2858989
9. Scaria, P.V. and Shafer, R.H. 1991. J. Biol. Chem. 266: 5417-5423. PMID: 2005088

Aspetto :
Powder
Stato fisico :
Solid
Solubilità :
water (10 mg/mL)
CONSERVAZIONE :
Store at room temperature
Punto di scioglimento :
260-262° C (lit.)(dec.)
Fluorescenza :
λex 480 nm, λem 620 nm in water
Solo per uso in Ricerca. Non previsto per Uso Diagnostico o Terapeutico.
WGK Germania :
3
RTECS :
SF7950000
PubChem CID :
Numero MDL :
MFCD00011724
Numero EC :
214-984-6
Registro Beilstein :
3642536
SMILES :
CC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[Br-]

Download SDS (MSDS)

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Ethidium bromide (CAS 1239-45-8)  Citazioni prodotti

Vedi quanti altri hanno usato Ethidium bromide (CAS 1239-45-8). Clicca sulla voce per laccesso a PubMed .

Ciatazioni 1 a10 di 12 totali

PMID: # 28565770  Ma, XW. et al. 2017. Exp Ther Med. 13: 1799-1805.

PMID: # 26811107  Pandurangan, M. et al. 2016. Biol Trace Elem Res. 173: 62-70.

PMID: # 26276565  Pandurangan, M. et al. 2016. Biol Trace Elem Res. 170: 309-19.

PMID: # 26985734  Pandurangan, M. et al. 2016. J. Photochem. Photobiol. B, Biol. 158: 206-11.

PMID: # 27176496  Zhang, M. et al. 2016. Mol Med Rep. 14: 121-8.

PMID: # 26519422  Pandurangan, M. et al. 2016. Biol Trace Elem Res. 171: 293-300.

PMID: # 23180761  Popuri, V. et al. 2013. Nucleic Acids Res. 41: 881-899.

PMID: # 23483870  Lian, LH. et al. 2013. Evid Based Complement Alternat Med. 2013: -.

PMID: # 23429911  Ikeda, E. et al. 2013. Mol. Pharmacol. 83: 959-967.

PMID: # 9657520  Kondo, S. et al. 1998. FASEB J. 12: 801-811.

Ciatazioni 1 a10 di 12 totali
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