Anticorpo EphA4 (D-4): sc-365503
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- Anti-EphA4 Antibody D-4 è un monoclonale di topo IgG2b κ EphA4 antibody, citato in 6 pubblicazioni, fornito in 200 µg/ml
- specifico per un epitopo mappato tra gli amminoacidi 889-921 vicino al C-terminus di EphA4 di origine human
- EphA4 Antibody (D-4) é raccomandato per il rilevamento di EphA4 di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA; anche reattivo con ulteriori specie, incluse e canine and porcine
- EphA4 Antibody (D-4) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
- disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
- disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
- Contattaci per ricevere un FREE 10 µg sample di EphA4 (D-4): sc-365503.
- m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for EphA4 Antibody (D-4) for WB and IHC(P) applications. This reagent is now offered in a bundle with EphA4 Antibody (D-4) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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What application is the blocking peptide sc-365503 P appropriate for?
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here:
https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Data di pubblicazione: 2017-02-24
Rated 5 di
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FM_CNRS da
Great antibody!
This antibody works extremely well for WB on mouse cells.
Data di pubblicazione: 2019-08-19
Rated 1 di
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Cathy Guo da
non-specific
Test IF with this antibody on both embryonic and early postnatal mouse brain tissue. No specific signal could be detected.
Data di pubblicazione: 2019-03-15
Rated 1 di
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Cathy Guo da
Not specific.
Test IF with this antibody on both embryonic and early postnatal mouse brain tissue. No specific signal could be detected.
Data di pubblicazione: 2019-03-15
Rated 4 di
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MS83 da
Good immunoblots in human oral epithelial cells
I tested this EphA4 antibody (free sample) in immuno blots. Our samples were human oral epithelial cells. The membrane was incubated with 1:1000 Ab in TBST-5% BSA at 4C for O/N. One band was detected at ~125 kDa. This antibody worked fine for us.
Data di pubblicazione: 2017-07-25
Rated 1 di
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John Chen da
This antibody does NOT work in our Western blot
We tested this antibody (free sample from SC) in Western Blot (WB) multiple times. Unfortunately, it does NOT work. Our samples were from mouse neuronal stem cells, which have been confirmed containing EphA4 in both IHC and WB by using other Abs. In our experiment, each lane was loaded by 100 ug total protein. The membrane was incubated with 1:1000 Ab in TBST-5% BSA at 4C for O/N. According to our results, there were multiple bands > 150 kDa , but no band at 110-120 kDa which is the expected Mw of EphA4.
Data di pubblicazione: 2016-12-20
Rated 4 di
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Steven Pelech at Kinexus da
Potent and fairly specific antibody
This monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was medium detection of the ~120 kDa target protein in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested, except for three major cross-reactive proteins of ~32, 48 and 62 kDa were also detected.
Data di pubblicazione: 2016-11-10
Rated 5 di
5
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SM da
Good publishable data in IF in chicken retina
Good publishable data in IF in chicken retina tissue. -SCBT Publication Review
Data di pubblicazione: 2015-04-25
Rated 5 di
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MS da
Produced excellent immunofluorescence membrane
Produced excellent immunofluorescence membrane staining in methanol-fixed HeLa cells. -SCBT QC
Data di pubblicazione: 2015-03-05