Dynein IC2, cytosolic Double Nickase Plasmid (h): sc-402079-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Dynein IC2, cytosolic Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Dynein IC2, cytosolic Double Nickase Plasmid (h) and Dynein IC2, cytosolic Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DYNC1I2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dynein IC2, cytosolic Double Nickase Plasmid (h) | sc-402079-NIC | 20 µg | $410.00 | |||
Dynein IC2, cytosolic Double Nickase Plasmid (h2) | sc-402079-NIC-2 | 20 µg | $410.00 |
DYNC1I2 encodes cytoplasmic dynein 1 intermediate chain 2 (Dynein IC2), a core subunit of the minus-end–directed microtubule motor complex that couples dynein to dynactin and diverse cargo adaptors. This motor system supports intracellular trafficking of endosomes, lysosomes, and Golgi-derived vesicles, as well as centrosome positioning, mitotic spindle organization, and retrograde transport needed for neuronal homeostasis. Dynein IC2 function integrates with microtubule-based transport, cell-cycle progression, and organelle dynamics pathways that shape polarity and proteostasis. Altered dynein–dynactin machinery has been implicated in neurodevelopmental and neurodegenerative phenotypes and other disorders linked to defective axonal transport and mitotic fidelity, making DYNC1I2 a useful node for mechanistic studies.
Dynein IC2, cytosolic Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DYNC1I2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DYNC1I2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DYNC1I2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DYNC1I2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.