Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Dnmt3L CRISPR/Cas9 KO Plasmid (m): sc-424871
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Dnmt3L CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Dnmt3L genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Dnmt3L Antibody (A-4): sc-393603
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dnmt3L CRISPR/Cas9 KO Plasmid (m) | sc-424871 | 20 µg | $397.00 |
Overview
Dnmt3l encodes Dnmt3L, a catalytically inactive cofactor that stimulates the de novo DNA methyltransferases DNMT3A and DNMT3B to establish and maintain DNA methylation patterns during development. In mouse, Dnmt3L is highly relevant to germline epigenetic programming, where it supports methylation of retrotransposons and imprint control regions, contributing to genome stability and parent-of-origin–specific gene regulation. Through these functions, Dnmt3L integrates into epigenetic regulation pathways that coordinate chromatin state, transcriptional silencing, and meiosis-associated reprogramming. Dysregulation of DNMT3L-associated methylation processes is linked to defects in imprinting, altered transposon repression, and broader epigenome instability that can influence developmental phenotypes and disease-relevant epigenetic states.
Dnmt3L CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Dnmt3l gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Dnmt3l together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Dnmt3l open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Dnmt3L protein expression.
This CRISPR knockout system enables efficient generation of Dnmt3l-deficient cell models for investigation of Dnmt3L signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Dnmt3l exon(s) critical for Dnmt3L function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Dnmt3l genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Dnmt3L CRISPR/Cas9 KO Plasmid (m) and Dnmt3L CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Dnmt3l locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Dnmt3L HDR Plasmid (m) and Dnmt3L HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Dnmt3l homology arms to support homology-directed repair at defined Dnmt3l target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.