Anticorpo demethylated-PP2A-C (4B7) raccomandato per il rilevamento di demethylated-PP2A-C di origine mouse, rat, human e Drosophila, Xenopus e S. pombe in WB, IP, IF e IHC(P)

Anticorpo demethylated-PP2A-C (4B7): sc-13601

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Anticorpo demethylated-PP2A-C (4B7) is rated 4.14 out of 5 by 14.
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Schede tecniche

    • Anti-demethylated-PP2A-C Antibody 4B7 è un monoclonale di topo IgG1 κ demethylated-PP2A-C antibody, citato in 33 pubblicazioni, fornito in 100 µg/ml
    • raised against the unmethylated C-terminal region of the PP2A-C subunit
    • demethylated-PP2A-C Antibody (4B7) é raccomandato per il rilevamento di demethylated-PP2A-C di origine mouse, rat, human e Drosophila, Xenopus e S. pombe in WB, IP, IF e IHC(P); to demethylate, treat with 100mM NaOH on ice
    • demethylated-PP2A-C Antibody (4B7) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • Contattaci per ricevere un FREE 10 µg sample di demethylated-PP2A-C (4B7): sc-13601.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for demethylated-PP2A-C Antibody (4B7) for WB and IHC(P) applications. These reagents are now offered in bundles with demethylated-PP2A-C Antibody (4B7) (see ordering information below).
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can I use it for ICC? PFA 4% fixed

Richiesta da: sherry z
Thank you for your question! Yes, this antibody is fine for use with immunocytochemistry. You can find our recommended protocols here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com.
Risposta da: Tech Service
Data di pubblicazione: 2017-03-08

Can demethylated-PP2A-C (4B7): sc-13601 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Richiesta da: jerojero
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, demethylated-PP2A-C (4B7): sc-13601 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Risposta da: Technical Support
Data di pubblicazione: 2017-02-24
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