DDT Antibody (1G1): sc-517061
- DDT Antibody (1G1) is a mouse monoclonal IgG2b κ DDT antibody provided at 100 µg/ml
- raised against amino acids 1-118 representing full length DDT of human origin
- recommended for detection of DDT of human origin by WB, IP and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for DDT Antibody (1G1). This work is in progress.
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DDT Antibody (1G1) is a mouse monoclonal IgG2b kappa light chain antibody that detects the DDT protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-DDT antibody (1G1) is available as the non-conjugated form. DDT, also known as D-dopachrome tautomerase, plays a crucial role in the inflammatory response, particularly in the context of liver function. DDT protein is primarily located in the cytoplasm as a homotrimer and is involved in the conversion of D-dopachrome into 5,6-dihydroxyindole, a key step in melanin biosynthesis. This process is significant because melanin acts as an antioxidant, helping to protect cells from oxidative stress. DDT protein is highly expressed in the liver, where DDT increases in response to liver damage, thereby enhancing melanin production to mitigate oxidative damage. Additionally, DDT′s interaction with macrophage migration inhibitory factor (MIF) suggests a broader role in modulating immune responses, making DDT protein an important target for studies related to inflammation and liver health.
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DDT Antibody (1G1) References:
- Crystal structure of human D-dopachrome tautomerase, a homologue of macrophage migration inhibitory factor, at 1.54 A resolution. | Sugimoto, H., et al. 1999. Biochemistry. 38: 3268-79. PMID: 10079069
- UVB-induced inflammation gives increased d-dopachrome tautomerase activity in blister fluid which correlates with macrophage migration inhibitory factor. | Sonesson, B., et al. 2003. Exp Dermatol. 12: 278-82. PMID: 12823441
- D-Dopachrome tautomerase is a candidate for key proteins to protect the rat liver damaged by carbon tetrachloride. | Hiyoshi, M., et al. 2009. Toxicology. 255: 6-14. PMID: 18930782
- Molecular cloning of a cDNA encoding a human macrophage migration inhibitory factor. | Weiser, WY., et al. 1989. Proc Natl Acad Sci U S A. 86: 7522-6. PMID: 2552447
- Purification, bioactivity, and secondary structure analysis of mouse and human macrophage migration inhibitory factor (MIF). | Bernhagen, J., et al. 1994. Biochemistry. 33: 14144-55. PMID: 7947826
- Cloning the human gene for macrophage migration inhibitory factor (MIF). | Paralkar, V. and Wistow, G. 1994. Genomics. 19: 48-51. PMID: 8188240
- NMR characterization of physicochemical properties of rat D-dopachrome tautomerase. | Yoshida, H., et al. 1997. Biochem Mol Biol Int. 42: 891-9. PMID: 9285056
- Molecular cloning of human D-dopachrome tautomerase cDNA: N-terminal proline is essential for enzyme activation. | Nishihira, J., et al. 1998. Biochem Biophys Res Commun. 243: 538-44. PMID: 9480844
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DDT Antibody (1G1) | sc-517061 | 100 µg/ml | $322.00 |