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Cytokeratin 18 Antibody (2X44): sc-70917

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Datasheets
  • Cytokeratin 18 Antibody (2X44) is a mouse monoclonal IgG1 κ, cited in 3 publications, provided at 200 µg/ml
  • raised against cytokeratins from the human bladder carcinoma cell line T24
  • recommended for detection of Cytokeratin 18 of human origin by WB, IP, IF, IHC(P) and FCM
  • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • See Cytokeratin 18 (RGE53): sc-32329 for Cytokeratin 18 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Cytokeratin 18 Antibody (2X44) for WB and IHC(P) applications. These reagents are now offered in bundles with Cytokeratin 18 Antibody (2X44) (see ordering information below).
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Cytokeratin 18 Antibody (2X44) is a mouse monoclonal IgG1 antibody that detects Cytokeratin 18 in human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Cytokeratin 18 is a vital component of the cytoskeleton in epithelial cells, contributing significantly to structural integrity and resilience. Cytokeratin 18 is predominantly located in the cytoplasm, forming a network of intermediate filaments that provide mechanical support and shape to cells. Proper localization of Cytokeratin 18 is crucial for maintaining epithelial tissue architecture, and disruption can lead to various pathological conditions, including cancer. Cytokeratin 18 serves as a biomarker for epithelial-derived tumors, as expression levels can indicate differentiation status of malignant cells. Post-translational modifications of Cytokeratin 18, such as phosphorylation, can influence interactions with other proteins and overall function within cells. Anti-Cytokeratin 18 antibody (2X44) enables researchers to explore epithelial biology and cancer pathology, advancing understanding of this protein′s role in health and disease.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Cytokeratin 18 Antibody (2X44)

sc-70917
200 µg/ml
$322.00

Cytokeratin 18 Antibody (2X44): m-IgG Fc BP-HRP Bundle

sc-539322
200 µg Ab; 10 µg BP
$361.00

Cytokeratin 18 Antibody (2X44): m-IgG1 BP-HRP Bundle

sc-541408
200 µg Ab; 20 µg BP
$361.00

Cytokeratin 18 Antibody (2X44) FITC

sc-70917 FITC
200 µg/ml
$336.00

Cytokeratin 18 Antibody (2X44) PE

sc-70917 PE
200 µg/ml
$349.00

Can Cytokeratin 18 (2X44): sc-70917 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: cjMara
Thank you for your question. Yes, Cytokeratin 18 (2X44): sc-70917 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-28
  • y_2026, m_5, d_20, h_12CST
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Rated 5 out of 5 by from Single strong band in MCF7 whole cell lysatesSingle strong band in MCF7 whole cell lysates. -SCBT QC
Date published: 2015-01-29
Rated 3 out of 5 by from Worked with human transfected lysate by WBWorked with human transfected lysate by WB -SCBT QC
Date published: 2015-01-12
Rated 4 out of 5 by from Positive WB data in Hep G2Positive WB data in Hep G2. -SCBT QC
Date published: 2015-01-02
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Cytokeratin 18 Antibody (2X44) is rated 4.0 out of 5 by 3.
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