Anticorpo cyclin B (D-1) raccomandato per il rilevamento di cyclin B di origine d in WB, IP, IF e ELISA

Anticorpo cyclin B (D-1): sc-166210

Anticorpo cyclin B (D-1) valutazione 4.1 di 5 di 9.
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Schede tecniche

    • Anti-cyclin B Antibody D-1 è un monoclonale di topo IgG1 κ cyclin B antibody, citato in 12 pubblicazioni, fornito in 200 µg/ml
    • generato contro gli amminoacidi 1-300 di cyclin B di origine Drosophila melanogaster
    • cyclin B Antibody (D-1) é raccomandato per il rilevamento di cyclin B di origine d in WB, IP, IF e ELISA
    • cyclin B Antibody (D-1) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • Contattaci per ricevere un FREE 10 µg sample di cyclin B (D-1): sc-166210.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for cyclin B Antibody (D-1) for WB applications. These reagents are now offered in bundles with cyclin B Antibody (D-1) (see ordering information below).
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What blocking solution should I use with cyclin B (D-1): sc-166210 monoclonal antibody for Western blot?

Richiesta da: jerojero
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Risposta da: Technical Support
Data di pubblicazione: 2017-04-13
  • y_2021, m_6, d_18, h_18CST
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Rated 5 di 5 di da Very specific antibody This antibody works well with my project! No non-specific bands.
Data di pubblicazione: 2020-02-24
Rated 4 di 5 di da Good features Good quality antibody for western blot detection of cyclin B in cell lysates with visible difference between proliferating and growth arrested cells
Data di pubblicazione: 2018-03-12
Rated 3 di 5 di da an OK antibody for WB This antibody generates a signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract), albeit the intensity is somewhat weak
Data di pubblicazione: 2017-09-18
Rated 4 di 5 di da not suitable for turbot there is a very light band when i exposed for 25min
Data di pubblicazione: 2017-07-10
Rated 1 di 5 di da Didnot work for WB No band was observed in MCF-7 cell lysates. Used the ab at 1:1000 dilution.
Data di pubblicazione: 2017-07-03
Rated 5 di 5 di da Great feature This antibody has been tested by WB for HepG2 cells. I used the product and diluted in TBST(1:200),4℃ overnight.
Data di pubblicazione: 2017-06-02
Rated 5 di 5 di da show good result In my Western blot analysis,this cyclinB antibody show good performance.The band is very strong.
Data di pubblicazione: 2016-12-05
Rated 5 di 5 di da It worked very nice on WB with Drosophila tissues It worked very nice on WB with Drosophila tissues.
Data di pubblicazione: 2015-05-19
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