Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CREM CRISPR/Cas9 KO Plasmid (h): sc-400406
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CREM CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CREM genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CREM Antibody (22): sc-101530
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CREM CRISPR/Cas9 KO Plasmid (h) | sc-400406 | 20 µg | $397.00 |
Overview
CREM (cAMP response element modulator) is a bZIP transcription factor in the CREB/ATF family that binds cAMP response elements to regulate stimulus-dependent gene expression programs. Through integration of cAMP/PKA signaling with calcium- and MAPK-linked inputs, CREM shapes transcriptional outcomes that influence cell proliferation, differentiation, and stress responses. CREM is particularly important in endocrine and reproductive biology, including regulation of spermatogenesis and neuroendocrine gene expression, and its isoform-specific activity can shift between transcriptional activation and repression. Dysregulated CREM signaling has been associated with altered immune and inflammatory transcriptional states and has been examined in contexts such as endocrine dysfunction, infertility-related mechanisms, and neuropsychiatric disease-relevant pathways.
CREM CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CREM gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CREM together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CREM open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CREM protein expression.
This CRISPR knockout system enables efficient generation of CREM-deficient cell models for investigation of CREM signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CREM exon(s) critical for CREM function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CREM genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CREM CRISPR/Cas9 KO Plasmid (h) and CREM CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CREM locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CREM HDR Plasmid (h) and CREM HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CREM homology arms to support homology-directed repair at defined CREM target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.