Cox-2 (h): 293 Lysate: sc-113099

Cox-2 (h): 293 Lysate is derived from the 293 cell line, a human embryonic kidney cell line known for its robust growth and high transfection efficiency. This lysate is enriched with human Cyclooxygenase-2 (Cox-2), an inducible enzyme that plays a pivotal role in the conversion of arachidonic acid to prostaglandins, which are involved in various physiological processes including inflammation, pain response, and the regulation of vascular functions. Unlike its constitutively expressed counterpart Cox-1, Cox-2 expression is typically induced by pro-inflammatory stimuli, cytokines, and growth factors. In research, Cox-2 (h): 293 Lysate is utilized to study the regulation, activity, and functional roles of Cox-2 in prostaglandin biosynthesis and inflammatory pathways. Researchers employ this lysate in Western blotting to detect and quantify Cox-2 expression levels under various experimental conditions, providing insights into its inducible nature and regulatory mechanisms. The lysate is also used in enzymatic activity assays to measure the conversion of arachidonic acid to prostaglandins, helping to explain the catalytic properties of Cox-2. Additionally, Cox-2 (h): 293 Lysate is valuable in co-immunoprecipitation assays to identify protein-protein interactions involving Cox-2, shedding light on its integration within larger signaling networks. Functional assays using this lysate can assess the impact of various stimuli on Cox-2 activity and its role in modulating inflammatory responses.