Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ceruloplasmin Double Nickase Plasmid (h): sc-401798-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ceruloplasmin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ceruloplasmin Double Nickase Plasmid (h) and ceruloplasmin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CP. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ceruloplasmin Antibody (H-3): sc-365205
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ceruloplasmin Double Nickase Plasmid (h) | sc-401798-NIC | 20 µg | $410.00 | |||
ceruloplasmin Double Nickase Plasmid (h2) | sc-401798-NIC-2 | 20 µg | $410.00 |
Human CP encodes ceruloplasmin, a multicopper ferroxidase that oxidizes Fe2+ to Fe3+ to facilitate transferrin loading and maintain systemic and cellular iron homeostasis. As a major copper-binding plasma protein, ceruloplasmin integrates copper transport with redox control and limits pro-oxidant iron chemistry that can amplify reactive oxygen species. CP activity influences extracellular antioxidant capacity and intersects with iron trafficking pathways in hepatocytes, macrophages, and cells of the central nervous system. Altered ceruloplasmin expression or function is associated with iron misdistribution and oxidative stress phenotypes relevant to neurodegeneration, metabolic dysregulation, and inflammatory states.
ceruloplasmin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CP locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CP. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CP function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CP-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.