Anticorpo CENP-B (C-10) raccomandato per il rilevamento di CENP-B di origine mouse, rat e human in WB, IP, IF e ELISA

Anticorpo CENP-B (C-10): sc-376392

Anticorpo CENP-B (C-10) valutazione 5.0 di 5 di 3.
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Schede tecniche

    • CENP-B Antibody C-10 è un monoclonale di topo IgG2a κ, citato in 2 pubblicazioni, fornito in 200 µg/ml
    • generato contro gli amminoacidi 535-599 mappati al C-terminus di CENP-B di origine human
    • CENP-B Antibody (C-10) é raccomandato per il rilevamento di CENP-B di origine mouse, rat e human in WB, IP, IF e ELISA
    • CENP-B Antibody (C-10) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • Contattaci per ricevere un FREE 10 µg sample di CENP-B (C-10): sc-376392.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred secondary detection reagent for CENP-B Antibody (C-10) for WB applications. This reagent is now offered in a bundle with CENP-B Antibody (C-10) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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What is the recommended fixation method for immunofluorescence staining with CENP-B (C-10): sc-376392 monoclonal antibody?

Richiesta da: Germaine
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Risposta da: Technical Support
Data di pubblicazione: 2017-02-24
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Rated 5 di 5 di da Good antibody for IF The CENP-B (C-10); sc-376392 performs well for IF showing nuclear localized staining pattern.
Data di pubblicazione: 2017-01-19
Rated 5 di 5 di da Produced great immunofluorescence nuclear Produced great immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Data di pubblicazione: 2015-04-24
Rated 5 di 5 di da Satisfactory Western Blot data of CENP Satisfactory Western Blot data of CENP-B expression in non-transfected and human CENP-B transfected 293T whole cell lysates. -SCBT QC
Data di pubblicazione: 2013-12-30
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