Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CDK5RAP1 CRISPR/Cas9 KO Plasmid (h): sc-407278
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CDK5RAP1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CDK5RAP1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CDK5RAP1 Antibody (D-1): sc-398764
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CDK5RAP1 CRISPR/Cas9 KO Plasmid (h) | sc-407278 | 20 µg | $397.00 |
Overview
CDK5RAP1 (CDK5 regulatory subunit–associated protein 1) is a radical S-adenosylmethionine enzyme localized primarily to mitochondria where it catalyzes post-transcriptional modification of mitochondrial tRNAs, supporting accurate mitochondrial translation and respiratory chain integrity. By regulating mitochondrial proteostasis and oxidative phosphorylation capacity, CDK5RAP1 influences cellular energy metabolism, redox balance, and stress-adaptive responses. Altered CDK5RAP1 activity has been linked to disrupted mitochondrial function and is frequently investigated in contexts of metabolic rewiring, hypoxia adaptation, and genome stability under oxidative stress. These pathways make CDK5RAP1 a relevant target for mechanistic studies in cancer biology and other disorders characterized by mitochondrial dysfunction.
CDK5RAP1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CDK5RAP1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CDK5RAP1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CDK5RAP1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CDK5RAP1 protein expression.
This CRISPR knockout system enables efficient generation of CDK5RAP1-deficient cell models for investigation of CDK5RAP1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CDK5RAP1 exon(s) critical for CDK5RAP1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CDK5RAP1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CDK5RAP1 CRISPR/Cas9 KO Plasmid (h) and CDK5RAP1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CDK5RAP1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CDK5RAP1 HDR Plasmid (h) and CDK5RAP1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CDK5RAP1 homology arms to support homology-directed repair at defined CDK5RAP1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.