Anticorpo Cdc45 (G-12) raccomandato per il rilevamento di Cdc45 di origine mouse, rat e human in WB, IP, IF e ELISA

Anticorpo Cdc45 (G-12): sc-55569

Anticorpo Cdc45 (G-12) valutazione 4.4 di 5 di 8.
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Schede tecniche

    • Anti-Cdc45 Antibody G-12 è un monoclonale di topo IgG3 κ Cdc45 antibody, citato in 19 pubblicazioni, fornito in 200 µg/ml
    • raised against amino acids 1-300 mapping at the N-terminus of Cdc45 of human origin
    • Cdc45 Antibody (G-12) é raccomandato per il rilevamento di Cdc45 di origine mouse, rat e human in WB, IP, IF e ELISA
    • Cdc45 Antibody (G-12) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • Contattaci per ricevere un FREE 10 µg sample di Cdc45 (G-12): sc-55569.
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for Cdc45 Antibody (G-12) for WB applications. This reagent is now offered in a bundle with Cdc45 Antibody (G-12) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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What is your recommended western blotting secondary antibody for use with Cdc45 (G-12): sc-55569?

Richiesta da: jenniferc
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Risposta da: Technical Support 4
Data di pubblicazione: 2017-02-04
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Rated 4 di 5 di da Worked in WB Worked fine at 1:500 in 10% milk, though you can see some non-specific bands.
Data di pubblicazione: 2017-08-30
Rated 1 di 5 di da Not useful for me It did not work in my hands. I saw a mix of bands, but none of them in the right place.
Data di pubblicazione: 2017-08-21
Rated 5 di 5 di da Reliable results! Cdc45 monoclonal antibody (G-12) gives consistent and reliable results in WB and has been validated against Cdc45 transfectants, and for endogenous detection.
Data di pubblicazione: 2017-01-19
Rated 5 di 5 di da Works well for IF applications Works well for IF applications, positive in methanol-fixed HeLa cells. -SCBT QC
Data di pubblicazione: 2015-04-04
Rated 5 di 5 di da Publication in WB Publication in WB, FCM and IF. Good data obtained with human osteosarcoma cell line U2OS. -SCBT Publication Review
Data di pubblicazione: 2015-04-02
Rated 5 di 5 di da WB: Detects endogenous Cdc45 in HeLa and WB: Detects endogenous Cdc45 in HeLa and Jurkat whole cell lysates. -SCBT QC
Data di pubblicazione: 2015-02-22
Rated 5 di 5 di da WB: Single strong band in A WB: Single strong band in A-431 nuclear extract. -SCBT QC
Data di pubblicazione: 2015-01-20
Rated 5 di 5 di da Worked with human transfected Cdc45 lysate by WB Worked with human transfected Cdc45 lysate by WB -SCBT QC
Data di pubblicazione: 2014-07-30
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