Cdc25A Antibody (DCS 121): sc-56263
- Cdc25A Antibody (DCS 121) is a mouse monoclonal IgG2a, cited in 1 publications, provided at 200 µg/ml
- raised against full length Cdc25A of human origin
- recommended for detection of Cdc25A of mouse, rat and human origin by WB, IP and ELISA
- See Cdc25A (F-6): sc-7389 for Cdc25A antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Cdc25A Antibody (DCS 121). This work is in progress.
Cdc25A Antibody (DCS 121) is a mouse monoclonal IgG2a antibody that detects Cdc25A in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Cdc25A plays a crucial role in the regulation of the cell cycle, particularly in the transition from G2 phase to mitosis, by acting as a phosphatase that dephosphorylates and activates cyclin-dependent kinases (CDKs). This activation is essential for the proper progression of mitosis, as cells move forward in the cycle and divide. Dysregulation of Cdc25A can lead to uncontrolled cell proliferation and is often implicated in various cancers, making Cdc25A a significant target for therapeutic intervention. Cdc25A is primarily located in the nucleus, where this phosphatase is involved in the regulation of mitotic entry, and nuclear localization is critical for function; any aberration in nuclear localization can disrupt the cell cycle and contribute to tumorigenesis. Cdc25A undergoes various post-translational modifications, including phosphorylation and ubiquitination, which can influence activity and stability, further underscoring Cdc25A′s importance in cell cycle regulation. anti-Cdc25A antibody (DCS 121) serves as an invaluable tool for researchers studying cell cycle dynamics and the molecular mechanisms underlying cell division and cancer.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Cdc25A Antibody (DCS 121) References:
- Cdc25A phosphatase: combinatorial phosphorylation, ubiquitylation and proteolysis. | Busino, L., et al. 2004. Oncogene. 23: 2050-6. PMID: 15021892
- CDC25A levels determine the balance of proliferation and checkpoint response. | Ray, D. and Kiyokawa, H. 2007. Cell Cycle. 6: 3039-42. PMID: 18073536
- CDC25A phosphatase: a rate-limiting oncogene that determines genomic stability. | Ray, D. and Kiyokawa, H. 2008. Cancer Res. 68: 1251-3. PMID: 18316586
- The role of Cdc25A in the regulation of cell proliferation and apoptosis. | Shen, T. and Huang, S. 2012. Anticancer Agents Med Chem. 12: 631-9. PMID: 22263797
- PKM2 dephosphorylation by Cdc25A promotes the Warburg effect and tumorigenesis. | Liang, J., et al. 2016. Nat Commun. 7: 12431. PMID: 27485204
- Ubiquitin-Specific Protease 29 Regulates Cdc25A-Mediated Tumorigenesis. | Chandrasekaran, AP., et al. 2021. Int J Mol Sci. 22: PMID: 34071237
- A novel CDC25A/DYRK2 regulatory switch modulates cell cycle and survival. | Lara-Chica, M., et al. 2022. Cell Death Differ. 29: 105-117. PMID: 34363019
- CDC25A inhibition suppresses cell proliferation and induces G1/S‑phase cell cycle arrest in nasopharyngeal carcinoma. | Wang, B., et al. 2023. Mol Med Rep. 27: PMID: 37052240
- Ginsenoside 20(S)-Rg3 reduces KIF20A expression and promotes CDC25A proteasomal degradation in epithelial ovarian cancer. | Zhang, R., et al. 2024. J Ginseng Res. 48: 40-51. PMID: 38223825
- Cryo-EM structure of the CDK2-cyclin A-CDC25A complex. | Rowland, RJ., et al. 2024. Nat Commun. 15: 6807. PMID: 39122719
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cdc25A Antibody (DCS 121) | sc-56263 | 200 µg/ml | $322.00 |