CD4 Inibitori per le analisi funzionali delle risposte cellulari a CD4
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The T cell receptor (TCR) is a heterodimer composed of either α and β or γ and δ chains. CD3 chains and the CD4 or CD8 co-receptors are also required for efficient signal transduction through the TCR. The TCR is expressed on T helper and T cytotoxic cells that can be distinguished by their expression of CD4 and CD8; T helper cells express CD4 proteins and T cytotoxic cells display CD8. CD4 is also expressed on cortical cells, mature medullary thymocytes, microglial cells and dendritic cells. CD4 (also designated T4 and Leu 3), is a membrane glycoprotein that contains four extracellular immunoglobin-like domains. The TCR in association with CD4 can bind class II MHC molecules presented by the antigen-presenting cells. The CD4 protein functions by increasing the avidity of the interaction between the TCR and an antigen-class II MHC complex. An additional role of CD4 is to function as a receptor for HIV.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
I would like to use CD4 (6A17): sc-70659 for immunoprecipitation. Which protein agarose do you recommend using with this antibody?
Richiesta da: cjMara
Thank you for your question. We recommend using Protein A/G PLUS-Agarose: sc-2003
Risposta da: Technical Support
Data di pubblicazione: 2017-04-12
Hello, I would like to know whether the anti-CD4 antibody (6A17) cat no sc-70659 needs a heat-mediated antigen retrieval for IHC-paraffin and if so what pH is recommended. Thank you
Richiesta da: Leonarda
Thank you for your inquiry. Yes, we do recommend antigen retrieval for IHC with paraffin-embedded sections. Heat treatment (recommended method): Place slides in a container and cover with 10 mM sodium citrate buffer, pH 6.0; or with 50 mM glycine-HCl buffer (glycine: sc-29096), pH 3.5, with 0.01% (w/v) EDTA (EDTA: sc-29092). Heat at 95° C for 5 minutes. Top off with fresh buffer and heat at 95° C for 5 minutes (optimal incubation time may vary for each tissue type). Allow slides to cool in the buffer for approximately 20 minutes. Wash in deionized H2O three times for 2 minutes each. Aspirate excess liquid from slides. You can view our full protocol here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
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