Anticorpo CD4 (6A17) raccomandato per il rilevamento di CD4 di origine human in WB, IP, IF e IHC(P)

Anticorpo CD4 (6A17): sc-70659

Anticorpo CD4 (6A17) valutazione 4.5 di 5 di 2.
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Schede tecniche

    • CD4 Antibody 6A17 è un monoclonale di topo IgG1 fornito in 250 µl supernatant
    • raised against the extracellular domain of CD4 of human origin
    • raccomandato per il rilevamento di CD4 di origine human in WB, IP, IF e IHC(P)
    • Vedere CD4 (MT310): sc-19641 for CD4 per anticorpi coniugati, tra cui AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 e 790.

I would like to use CD4 (6A17): sc-70659 for immunoprecipitation. Which protein agarose do you recommend using with this antibody?

Richiesta da: cjMara
Thank you for your question. We recommend using Protein A/G PLUS-Agarose: sc-2003 https://www.scbt.com/scbt/product/protein-a-g-plus-agarose
Risposta da: Technical Support
Data di pubblicazione: 2017-04-12

Hello, I would like to know whether the anti-CD4 antibody (6A17) cat no sc-70659 needs a heat-mediated antigen retrieval for IHC-paraffin and if so what pH is recommended. Thank you

Richiesta da: Leonarda
Thank you for your inquiry. Yes, we do recommend antigen retrieval for IHC with paraffin-embedded sections. Heat treatment (recommended method): Place slides in a container and cover with 10 mM sodium citrate buffer, pH 6.0; or with 50 mM glycine-HCl buffer (glycine: sc-29096), pH 3.5, with 0.01% (w/v) EDTA (EDTA: sc-29092). Heat at 95° C for 5 minutes. Top off with fresh buffer and heat at 95° C for 5 minutes (optimal incubation time may vary for each tissue type). Allow slides to cool in the buffer for approximately 20 minutes. Wash in deionized H2O three times for 2 minutes each. Aspirate excess liquid from slides. You can view our full protocol here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Risposta da: Technical Service
Data di pubblicazione: 2016-11-02
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Rated 4 di 5 di da Worked with transfected lysate by WB Worked with transfected lysate by WB -SCBT QC
Data di pubblicazione: 2015-03-31
Rated 5 di 5 di da Works well for WB with SUP Works well for WB with SUP-T1 and CCRF-CEM whole cell lysates. -SCBT QC
Data di pubblicazione: 2013-10-13
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