CD15 Antibody (C3D-1): sc-19648

CD15 Antibody (C3D-1) is a mouse monoclonal IgM antibody that detects CD15 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). CD15 (C3D-1) antibody is available in both non-conjugated and various conjugated forms, including Alexa Fluor® 488 conjugate. CD15, also known as Lewis X or LeX, is a carbohydrate antigen synthesized by Fucosyltransferase IV (FucT-IV), which catalyzes the addition of fucose to sugar acceptor molecules, resulting in the formation of a trisaccharide crucial for cell surface interactions. CD15 is predominantly located on the surface of leukocytes and certain tumor cells, where CD15 plays a vital role as an adhesion molecule, facilitating calcium-mediated homotypic binding. CD15 presence on the cell surface promotes strong self-aggregation among cells, enhancing their ability to interact with one another in the presence of calcium ions. CD15 is recognized as a ligand for selectins, which are essential for mediating leukocyte-specific cellular interactions, thereby underscoring CD15's importance in immune response and inflammation. CD15's ability to mediate these interactions makes CD15 a significant target for research in immunology and cancer biology, as understanding CD15's function can provide insights into cellular adhesion processes and potential therapeutic interventions.

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CD15 Antibody (C3D-1) References:

1. Role of the Lewis(x) glycan determinant in corneal epithelial cell adhesion and differentiation.  |  Cao, Z., et al. 2001. J Biol Chem. 276: 21714-23. PMID: 11278542
2. Le(X) and related structures as adhesion molecules.  |  Hakomori, S. 1992. Histochem J. 24: 771-6. PMID: 1362193
3. Mice lacking alpha1,3-fucosyltransferase IX demonstrate disappearance of Lewis x structure in brain and increased anxiety-like behaviors.  |  Kudo, T., et al. 2007. Glycobiology. 17: 1-9. PMID: 16973732
4. Binding of the adhesion and pathogen receptor DC-SIGN by monocytes is regulated by the density of Lewis X molecules.  |  Gijzen, K., et al. 2007. Mol Immunol. 44: 2481-6. PMID: 17175026
5. Characteristic expression of Lewis-antigenic glycolipids in human ovarian carcinoma-derived cells with anticancer drug-resistance.  |  Iwamori, M., et al. 2007. J Biochem. 141: 309-17. PMID: 17190787
6. Specific interaction between Lex and Lex determinants. A possible basis for cell recognition in preimplantation embryos and in embryonal carcinoma cells.  |  Eggens, I., et al. 1989. J Biol Chem. 264: 9476-84. PMID: 2470757
7. CD15 immunostaining improves placental diagnosis of fetal hypoxia.  |  Seidmann, L., et al. 2021. Placenta. 105: 41-49. PMID: 33545630
8. CD15+ Bone Marrow-derived Cells Are Regulators of Immune Response in ARG1-producing Colorectal Cancer Cells.  |  Miyoshi, K., et al. 2022. Anticancer Res. 42: 459-470. PMID: 34969756
9. Prognostic and Therapeutic Role of CD15 and CD15s in Cancer.  |  Szlasa, W., et al. 2022. Cancers (Basel). 14: PMID: 35565333
10. Peripheral T-cell lymphomas expressing CD30 and CD15 expand the spectrum of anaplastic large cell lymphoma, ALK-negative.  |  Ganapathi, KA., et al. 2024. Br J Haematol. 204: 1862-1871. PMID: 38613165
11. A carbohydrate structure associated with CD15 (Lewis x) on myeloid cells is a novel ligand for human CD2.  |  Warren, HS., et al. 1996. J Immunol. 156: 2866-73. PMID: 8609406
12. A combination of CD34 selection and complement-mediated immunopurging (anti-CD15 monoclonal antibody) eliminates tumor cells while sparing normal progenitor cells.  |  Nimgaonkar, M., et al. 1996. J Hematother. 5: 39-48. PMID: 8646480

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD15 Antibody (C3D-1) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-CD15 Antibody (C3D-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD15 Antibody (C3D-1) Specifications

Alternate Product Name: FUT4

Product Clone Name: C3D-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids:

Epitope Target: CD15

Epitope Species: human

Product Reactivity/Detection: CD15

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for CD15 Antibody (C3D-1): AF488: Alexa Fluor^® 488