caspase-1 p20 Antibody D-4 è un monoclonale di topo IgG2b κ, citato in 43 pubblicazioni, fornito in 200 µg/ml
specifico per un epitopo mappato tra gli amminoacidi 259-294 al C-terminus di caspase-1 p20 di origine mouse
caspase-1 p20 Antibody (D-4) é raccomandato per il rilevamento di caspase-1 precursor and p20 subunit di origine mouse e rat in WB, IP, IF, IHC(P) e ELISA
caspase-1 p20 Antibody (D-4) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
Contattaci per ricevere un FREE 10 µg sample di caspase-1 p20 (D-4): sc-398715.
m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) is the preferred secondary detection reagent for caspase-1 p20 Antibody (D-4) for WB and IHC(P) applications. This reagent is now offered in a bundle with caspase-1 p20 Antibody (D-4) (see ordering information below).
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Caspase-1, originally designated ICE (for IL-1 converting enzyme), is a member of the group of caspases with large prodomains. Caspase-1 promotes maturation of interleukin IL-1 beta and interleukin18 (IL-18) by proteolytic cleavage of precursor forms into biologically active pro-inflamatory cytokines. The prodomain of caspase-1 (also known as Pro-C1) represents the amino acid terminal portion of the caspase-1 precursor. Pro-C1 is produced as a residual component after proteolytic cleavage of the precursor generates the functional caspase-1 subunits known as the p20 and p10 subunits. Active caspase-1, a (p20/p10)2 tetramer, is necessary and sufficient for cleavage of precursor IL-1 as well as for induction of apoptosis in some cell lines. The highly conserved family of caspases mediate many of the morphological and biochemical features of apoptosis, including structural dismantling of cell bodies and nuclei, fragmentation of genomic DNA, destruction of regulatory proteins and propagation of other pro-apoptotic molecules. The human Caspase-1 gene maps to chromosome 2q14 and encodes a cytoplasmic protein expressed in liver, heart, skeletal muscle kidney and testis. Caspase-1 has been implicated in inflammation, septic shock, and other situations such as wound healing and the growth of certain leukemias.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What is the expected molecular weight of the protein detected by this antibody, caspase-1 p20 (D-4): sc-398715?
Richiesta da: TinTin
This antibody, caspase-1 p20: sc-398715, is recommended for the detection of caspase-1 precursor and p20 subunit. The expected molecular weights of these caspase forms are ~45kDa and ~20kDa, respectively, however detection of the p20 subunit can be difficult to observe by Western blot, so it is more common to only see the precursor band.
Risposta da: Technical Support 12
Data di pubblicazione: 2017-01-24
What application is the blocking peptide sc-398715 P appropriate for?
Richiesta da: jenni
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Rated 5 di
功能出色同事订购过此产品caspase-1 p20抗体 (D-4)，使用效果不错，条带清晰，我希望可以获取小样试用，将会非常高兴。
Data di pubblicazione: 2018-05-11
Rated 5 di
qin ziying01 da
IHCUsed it for IHC got very perfect staining for mouse colon section.1:150 very good concetration.
Data di pubblicazione: 2018-01-27
Rated 1 di
Caspase-1 p20 antibodyI received a sample size to test for active caspase-1 in the medium of BMDCs stimulated in various conditions. I wasn't able to detect any bands at all. :(
Data di pubblicazione: 2018-01-06
Rated 5 di
Good!Caspase 1 p-20(d-4) protein: from mouse liver extracted with RIPA
Data di pubblicazione: 2017-12-12
Rated 5 di
excellent qualityA good staining was obtained in rat colon tissue in IHC(1:100 dilution) and very good antibodies were obtained.
Data di pubblicazione: 2017-10-26
Rated 5 di
功能很好上次申请的小样试用装非常好用，它不仅能曝出全长的caspase 1蛋白条带，还能曝出caspase-1 p20条带。但是就是还有些扎蛋白条带。不过都不影响条带。
Data di pubblicazione: 2017-10-15
Rated 3 di
Trial Report sc-398715I bought this antibody(sc-398715) a month ago and it's pity that there was no flaser. Maybe the protein I dectected was not form the model organism-mouse so there are many different amino acid sequences in this protein.
Data di pubblicazione: 2017-09-25
Rated 5 di
The antibody had high sensitivityWe got obtained specific band is very clear and powerful signal.Expressions were much higher and more widely used.
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