Myc (h2): 293T Lysate: sc-116671

c-Myc (h2): 293T Lysate, derived from the HEK 293T cell line, is a valuable resource for studying the c-Myc protein, a pivotal transcription factor involved in regulating cell proliferation, growth, and apoptosis. The HEK 293T cells, a modified variant of the original HEK 293 line, are engineered to express the SV40 large T antigen, enhancing their ability to produce high levels of recombinant proteins. This makes them particularly useful for generating lysates with abundant c-Myc, facilitating detailed biochemical and molecular analyses. Researchers use c-Myc (h2): 293T Lysate to investigate the mechanisms by which c-Myc regulates gene expression. c-Myc binds to specific DNA sequences known as E-boxes and recruits various co-factors to activate or repress the transcription of target genes involved in cell cycle progression and metabolism. Techniques such as chromatin immunoprecipitation (ChIP), Western blotting, and reporter assays are commonly employed to study c-Myc's interaction with DNA and its effect on transcriptional regulation. This lysate is instrumental in exploring the signal transduction pathways and cellular processes controlled by c-Myc, providing insights into its role in fundamental biological functions.