Anticorpo beta 3 Tubulin (AA10) raccomandato per il rilevamento di β3 Tubulin di origine mouse, rat e human in WB, IP e IF

Anticorpo β3 Tubulin (AA10): sc-80016

Anticorpo β3 Tubulin (AA10) valutazione 4.8 di 5 di 4.
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Schede tecniche

    • beta 3 Tubulin Antibody AA10 è un monoclonale di topo IgG2a κ, citato in 31 pubblicazioni, fornito in 200 µg/ml
    • raised against β3 Tubulin of rat origin, with epitope mapping to amino acids 436-450
    • beta 3 Tubulin Antibody (AA10) é raccomandato per il rilevamento di β3 Tubulin di origine mouse, rat e human in WB, IP e IF
    • beta 3 Tubulin Antibody (AA10) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • Contattaci per ricevere un FREE 10 µg sample di β3 Tubulin (AA10): sc-80016.
    • m-IgG2a BP-HRP is the preferred secondary detection reagent for β3 Tubulin Antibody (AA10) for WB applications. This reagent is now offered in a bundle with β3 Tubulin Antibody (AA10) (see ordering information below).
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I need to use an antibody to detect the protein in samples of porcine origin. Do you know if this mouse monoclonal antibody will work?

Richiesta da: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Risposta da: Tech Service 3
Data di pubblicazione: 2017-02-01
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Rated 4 di 5 di da Melanocortin 4 receptor activation protects striat α-Melanocyte stimulating hormone (α-MSH) is a melanocortin which exerts potent anti-inflammatory and antiapoptotic effects. Melanocortin 4 receptors (MC4R) are abundantly expressed in the brain and we previously demonstrated that [Nle(4), D-Phe(7)]melanocyte-stimulating hormone (NDP-MSH), an α-MSH analogue, increased expression of brain derived-neurotrophic factor (BDNF), and peroxisome proliferator-activated receptor- γ (PPAR-γ). We hypothesized that melanocortins could affect striatal cell survival through BDNF and PPAR-γ. First, we determined the expression of these factors in the striatum. Acute intraperitoneal administration (0.5 mg/kg) of α-MSH increased the levels of BDNF mRNA in rat striatum but not in rat cerebral cortex. Also, protein expression of PPAR-γ and MC4R was increased by acute treatment with α-MSH in striatum but not in cortex. No changes were observed by 48 h treatment. Next, we evaluated melanocortins effect on neuron and glial survival. 3-nitropropionic acid (3-NP), which is known to induce striatal degeneration, was used to induce cell death in the rat striatal cell line ST14A expressing mutant human huntingtin (Q120) or in ST14A cells expressing normal human huntingtin (Q15), in primary cultured astrocytes, and in BV2 cells. NDP-MSH protected Q15 cells, astrocytes and BV2 cells from death by 3-NP whereas it did not fully protect Q120 cells. Protection of Q15 cells and astrocytes was blocked by a MC4R specific inhibitor (JKC-363) and a PPAR-γ antagonist (GW9662). The BDNF receptor antagonist (ANA-12) abolished NDP-MSH protective effect in astrocytes but not in Q15 cells. We demonstrate for the first time that melanocortins, acting through PPAR-γ and BDNF, protect neurons and glial cells from 3-NP toxicity
Data di pubblicazione: 2018-12-13
Rated 5 di 5 di da Very good result beta-3-tubulin antibody (AA10) sc-800166: Immunofluorescence staining of beta-3-tubulin (green) and S100 (red) in rat dorsal root ganglion fixed in 4% paraformaldehyde.
Data di pubblicazione: 2018-02-06
Rated 5 di 5 di da Produced good western blot band The tubulin antibody produced a clear western blot band for our cell lysate
Data di pubblicazione: 2016-12-20
Rated 5 di 5 di da Great antibody for Western Great antibody for Western, strong tubulin band detected in A-431, K-562 and PC-12 whole cell lysates. -SCBT QC
Data di pubblicazione: 2013-10-22
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