Anticorpo beta-Arrestin-2 (D-5) raccomandato per il rilevamento di β-Arrestin-2 di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA

Anticorpo β-Arrestin-2 (D-5): sc-166935

Anticorpo β-Arrestin-2 (D-5) valutazione 5.0 di 5 di 4.
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Schede tecniche

    • beta-Arrestin-2 Antibody D-5 è un monoclonale di topo IgG1 κ, citato in 1 pubblicazioni, fornito in 200 µg/ml
    • generato contro gli amminoacidi 78-125 mappati vicino al N-terminus di β-Arrestin-2 di origine human
    • raccomandato per il rilevamento di β-Arrestin-2 di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA
    • Vedere β-Arrestin-2 (B-4): sc-365445 for β-Arrestin-2 per anticorpi coniugati, tra cui AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 e 790.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for β-Arrestin-2 Antibody (D-5) for WB and IHC(P) applications. These reagents are now offered in bundles with β-Arrestin-2 Antibody (D-5) (see ordering information below).
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I am using β-Arrestin-2 (D-5): sc-166935 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Richiesta da: Cweed
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Risposta da: Technical Support
Data di pubblicazione: 2017-02-24
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Rated 5 di 5 di da Various expression levels of various cell Various expression levels of various cell lines demonstrated by WB - SK-N-MC, HepG2, T84, NTERA-2 cl.D1 and A549 whole cell lysates. -SCBT QC
Data di pubblicazione: 2015-04-25
Rated 5 di 5 di da Positive detection of the Positive detection of the -Arrestin-2 protein by WB using mouse PBL whole cell lysate. -SCBT QC
Data di pubblicazione: 2015-02-08
Rated 5 di 5 di da Excellent immunofluorescence nuclear staining Excellent immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
Data di pubblicazione: 2015-02-01
Rated 5 di 5 di da An increase in signal was observed via Western An increase in signal was observed via Western Blot when comparing 293T cells transfected to over express human -Arrestin-2 with those that have not. -SCBT QC
Data di pubblicazione: 2015-01-20
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