Anticorpo beta-Arrestin-1/2 (A-1) raccomandato per il rilevamento di β-Arrestin-1 and β-Arrestin-2 di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA

Anticorpo β-Arrestin-1/2 (A-1): sc-74591

Anticorpo β-Arrestin-1/2 (A-1) valutazione 4.7 di 5 di 16.
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Schede tecniche

    • Anti-beta-Arrestin-1/2 Antibody A-1 è un monoclonale di topo IgG1 κ β-Arrestin-1/2 antibody, citato in 17 pubblicazioni, fornito in 200 µg/ml
    • generato contro aminoacidi 7-290 mapping vicino il N-terminus del β-Arrestin-1 di origine human
    • beta-Arrestin-1/2 Antibody (A-1) é raccomandato per il rilevamento di β-Arrestin-1 and β-Arrestin-2 di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA; anche reattivo con ulteriori specie, incluse porcine
    • beta-Arrestin-1/2 Antibody (A-1) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • Contattaci per ricevere un FREE 10 µg sample di β-Arrestin-1/2 (A-1): sc-74591.
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for β-Arrestin-1/2 Antibody (A-1) for WB and IHC(P) applications. This reagent is now offered in a bundle with β-Arrestin-1/2 Antibody (A-1) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Richiesta da: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Risposta da: Technical Support 15
Data di pubblicazione: 2017-01-25
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Rated 5 di 5 di da KO検証しました 正常細胞とbeta-arrestin1/2 KO細胞で検証したところ、正常細胞でしかバンドが出ませんでした。特異性が高い抗体だと思います
Data di pubblicazione: 2021-11-30
Rated 5 di 5 di da This antibody was good This antibody was used to detect the ARRB1 protein and ARRB2 protein in ARRB1-overexpressed A549 cells and ARRB2-overexpressed A549 cells
Data di pubblicazione: 2019-12-04
Rated 5 di 5 di da Endogenous and recombinant beta-arrestin1 in WB/IF Product sc-74591 detected a unique band in non-transfected HEK293 cells and an additional band corresponding to transfected beta-arrestin1 recombinant proteins when used at 1:600 ratio followed by fluorescent secondary antibody detection IRDye 800CW used at 1:10,000 ratio. It did not detect recombinant beta-arrestin2 overexpressed in HEK293T cells. Immunocytochemistry gave a cytoplasmic staining with a slight nuclear staining when used at 1:200 ratio in HEK293T cells.
Data di pubblicazione: 2019-04-26
Rated 4 di 5 di da good 1/400 dilution was used for WB, specific band was shown.
Data di pubblicazione: 2018-12-24
Rated 5 di 5 di da Great antibody! It is a very good antibody for both western blots and IP!
Data di pubblicazione: 2018-10-28
Rated 5 di 5 di da Good for IF This β-Arrestin-1/2 antibody shows strong specific signal in IF staining in primary rat motor neuron.
Data di pubblicazione: 2017-11-06
Rated 4 di 5 di da β-Arrestin-1/2 Antibody western We have received a free sample of th bArresting 1/2 antibody. I am satisfied with the western blot, it worked the way it is shown in the picture.
Data di pubblicazione: 2017-07-03
Rated 3 di 5 di da Try and install the response WB stripe is not clear Difficult to raise,WB strips are less miscellaneous,
Data di pubblicazione: 2017-06-28
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