Benzonase® Nuclease Proteina biologicamente attiva: una endonucleasi ideale per applicazioni in cui è desiderabile una completa digestione degli acidi nucleici

Benzonase® Nuclease (CAS 9025-65-4)

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Benzonase® Nuclease is rated 5.0 out of 5 by 2.
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Nomi alternativi: BenzNuclease
Applicazione: Benzonase® Nuclease supplied in solution containing 20 mM Tris HCl (pH 8.0), 2 mM MgCl2, 20 mM NaCl and 50% glycerol is an endonuclease ideal for applica
Numero CAS: 9025-65-4
Purezza: ≥90%
Solo per uso in Ricerca. Non previsto per Uso Diagnostico o Terapeutico.
* Vedere Certificato di Analisi per informazioni sul lotto specifico (incluso il contenuto d'acqua).
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Benzonase® Nuclease is a solubilized version of Benzonase® Nuclease Lyophilized Powder (sc-391121). The Benzonase® enzyme is a genetically engineered endonuclease from Serratia marcescens. Benzonase® Nuclease, also called Golden nuclease, is a highly useful endonuclease for removal of nucleic acids from recombinant proteins and protein fragments, and is also used for the reduction of viscosity in protein extracts.

Benzonase® Nuclease is a genetically engineered endonuclease from Serratia marcescens. Benzonase® Nuclease, also called Golden nuclease, is a highly useful endonuclease for removal of nucleic acids from recombinant proteins and protein fragments, and is also used for the reduction of viscosity in protein extracts.

The enzyme completely digests nucleic acids to 5′-monophosphate terminated oligonucleotides 2–5 bases in length. Although the nuclease is capable of cleavage at nearly all positions along a nucleic acid chain, sequence-dependent preferences have been demonstrated. The enzyme prefers GC-rich regions in dsDNA while avoiding d(A)/d(T)-tracts. Benzonase is ideal for a wide variety of applications where complete digestion of nucleic acids is desirable. Digests native or heat-denatured DNA and RNA. This can also be used for the removal of nucleic acid from protein samples.

Unit definition:
One unit of Benzonase Nuclease is defined as the amount of enzyme that causes a ΔA260 of 1.0 in 30 min, which corresponds to complete digestion of 37 μg of DNA. Note that 1 KU = 1000 units.

Benzonase is a registered trademark of Merck KGaA.


Referenze

1. Miller, M.D., et al. 1994. Nat. Struct. Biol. 1: 461-468. PMID: 7664065

2. Friedhoff, P., et al. 1994. Nucleic Acids Res. 22: 3280-3287. PMID: 8078761

3. Ball, T.K., et al. 1992. Nucleic Acids Res. 20: 4971-4974. PMID: 1329033

4. Ball, T.K., et al. 1987. Gene. 57: 183-192. PMID: 3319779

5. Janning, P., et al. 1994. Rapid Commun. Mass Spectrom. 8: 1035-1040. PMID: 7696699

6. Molloy, M.P., et al. 1998. Electrophoresis. 19: 837-844. PMID: 9629924

7. Herbert, B.R., et al. 1998. Electrophoresis. 19: 845-851. PMID: 9629925

Utilizzo :
The activity of any unknown nuclease can be determined from a single measurement by means of the standard curve. The specific activity of Benzonase® Nuclease is 1.3×106 ~ 1.5×106 unit/mg protein.
Formulazione :
≥250 units/µL supplied in solution containing 20 mM Tris HCl (pH 8.0), 2 mM MgCl2, 20 mM NaCl and 50% glycerol.
Stato fisico :
Liquid
Derivato da :
Recombinant protein expressed in Escherichia coli
CONSERVAZIONE :
Store at -20° C
Solo per uso in Ricerca. Non previsto per Uso Diagnostico o Terapeutico.
WGK Germania :
1
Numero MDL :
MFCD00131010
Numero EC :
231-791-2

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CERTIFICATO DI ANALISI

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Benzonase® Nuclease (CAS 9025-65-4)  Citazioni prodotti

Vedi quanti altri hanno usato Benzonase® Nuclease (CAS 9025-65-4). Clicca sulla voce per laccesso a PubMed .

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PMID: # 33930289  Mendel, M.|Delaney, K.|Pandey, RR.|Chen, KM.|Wenda, JM.|Vågbø, CB.|Steiner, FA.|Homolka, D.|Pillai, RS.| et al. 2021. Cell.

PMID: # 32611815  Dong, C.|West, KL.|Tan, XY.|Li, J.|Ishibashi, T.|Yu, CH.|Sy, SMH.|Leung, JWC.|Huen, MSY.| et al. 2020. Proc Natl Acad Sci U S A. 117: 17019-17030.

PMID: # 31263572  Keskitalo, S.|Haapaniemi, EM.|Glumoff, V.|Liu, X.|Lehtinen, V.|Fogarty, C.|Rajala, H.|Chiang, SC.|Mustjoki, S.|Kovanen, P.|Lohi, J.|Bryceson, YT.|Seppänen, M.|Kere, J.|Heiskanen, K.|Varjosalo, M.| et al. 2019. NPJ Genom Med. 4: 14.

PMID: # 30692695  Charlesworth, CT.|Deshpande, PS.|Dever, DP.|Camarena, J.|Lemgart, VT.|Cromer, MK.|Vakulskas, CA.|Collingwood, MA.|Zhang, L.|Bode, NM.|Behlke, MA.|Dejene, B.|Cieniewicz, B.|Romano, R.|Lesch, BJ.|Gomez-Ospina, N.|Mantri, S.|Pavel-Dinu, M.|Weinberg, KI.|Porteus, MH.| et al. 2019. Nat. Med. 25: 249-254.

PMID: # 31135337  Bigot, N.|Day, M.|Baldock, RA.|Watts, FZ.|Oliver, AW.|Pearl, LH.| et al. 2019. Elife. 8:

PMID: # 31163614  Villafañez, F.|Gottifredi, V.|Soria, G.| et al. 2019. High Throughput. 8:

PMID: # 29565900  van Rooden, EJ.|Florea, BI.|Deng, H.|Baggelaar, MP.|van Esbroeck, ACM.|Zhou, J.|Overkleeft, HS.|van der Stelt, M.| et al. 2018. Nat Protoc. 13: 752-767.

PMID: # 30205044  Archer, TC.|Ehrenberger, T.|Mundt, F.|Gold, MP.|Krug, K.|Mah, CK.|Mahoney, EL.|Daniel, CJ.|LeNail, A.|Ramamoorthy, D.|Mertins, P.|Mani, DR.|Zhang, H.|Gillette, MA.|Clauser, K.|Noble, M.|Tang, LC.|Pierre-François, J.|Silterra, J.|Jensen, J.|Tamayo, P.|Korshunov, A.|Pfister, SM.|Kool, M.|Northcott, PA.|Sears, RC.|Lipton, JO.|Carr, SA.|Mesirov, JP.|Pomeroy, SL.|Fraenkel, E.| et al. 2018. Cancer Cell. 34: 396-410.e8.

PMID: # 29303417  Farrokhi, A.|Pakyari, M.|Nabai, L.|Pourghadiri, A.|Hartwell, R.|Jalili, R.|Ghahary, A.| et al. 2018. Tissue Eng Part A. 24: 955-967.

PMID: # 30154153  Högström, J.|Heino, S.|Kallio, P.|Lähde, M.|Leppänen, VM.|Balboa, D.|Wiener, Z.|Alitalo, K.| et al. 2018. Cancer Res. 78: 5820-5832.

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