Anticorpo Beclin-1/BECN1 (E-8): sc-48341
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- Anti-Beclin-1/BECN1 Antibody E-8 è un monoclonale di topo IgG2a κ Beclin-1/BECN1 antibody, citato in 151 pubblicazioni, fornito in 200 µg/ml
- generato contro gli amminoacidi 1-300 di BECN1 di origine human
- Beclin-1/BECN1 Antibody (E-8) é raccomandato per il rilevamento di Beclin 1 di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA
- Beclin-1/BECN1 Antibody (E-8) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
- disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
- disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
- Contattaci per ricevere un FREE 10 µg sample di Beclin-1/BECN1 (E-8): sc-48341.
- m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for Beclin-1/BECN1 Antibody (E-8) for WB and IHC(P) applications. These reagents are now offered in bundles with Beclin-1/BECN1 Antibody (E-8) (see ordering information below).
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- Anticorpi Beclin
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Is this product suitable for use in flow cytometry? Is there a protocol if it is convenient to use? Thanks for help.
Thank you for your question. We recommend the AlexaFluor conjugates of this antibody for flow cytometry. You can find our recommended protocol for flow cytometry by following this link:
https://www.scbt.com/scbt/de/resources/protocols/flow-cytometry
Data di pubblicazione: 2018-12-29
What dilution has worked well with rat brain samples?
Thank you for your question. We do not have any data in rat brain samples specifically when using this mouse monoclonal antibody, BECN1 (E-8): sc-48341. We used this primary at a dilution of 1:200 for most of the Western blot data on our website. We recommend a starting dilution of 1:200 (range of 1:100-1:1,000). A link to our recommended protocol is provided below.
https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Data di pubblicazione: 2017-06-14
What is the protocol for immunohistochemistry? I have found the support products, but not the protocol.
Thank you for your question. Here is a link to the IHC protocol:
https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
If you have any further questions, please feel free to contact Technical Support by phone, email or chat.
Data di pubblicazione: 2020-08-11
If I freeze this antibody, will it remain viable for a longer period of time?
Thank you for your question. We do not run long term storage testing, but warranty all of our products for 1 year from the date of purchase. Freezing the antibody will actually void the warranty as this harms the antibody due to the presence of gelatin in the suspension mixture. The recommended storage temperature is 4ºC and many times the antibody will remain viable under these conditions for much longer than the 1 year warranty date. If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Data di pubblicazione: 2016-12-30
Rated 4 di
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Markov da
Good results
This antibody works good at 1:1000 in colon cancer cell lines
Data di pubblicazione: 2020-12-02
Rated 5 di
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calu da
Good signal in WB
HepG2 cells, dilution 1:1000.
Strong and clear signal, without specific bands.
Data di pubblicazione: 2020-09-11
Rated 5 di
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Shuiqiao da
good antibody
using 20ug mouse brain lysate it worked well in 1:1000 dilution.
Data di pubblicazione: 2020-07-02
Rated 5 di
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erik da
Good results
1:600 diluted for wb of HepG2 cell lysis, and the band is strong .
Data di pubblicazione: 2018-12-24
Rated 5 di
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Mao123 da
very good antibody for western blotting
Dlituion 1:1000
cells: 16:HBE
Strong and specific band
Data di pubblicazione: 2018-12-19
Rated 5 di
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doctorwn da
Great antibody
I used it with 1:1000 dilution in both human cardiomyocytes and mouse fibroblasts. Got very strong and clear bands.
Data di pubblicazione: 2018-10-10
Rated 5 di
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cuci55 da
really good
I tested this antibody this week, the outcome is really great.
Data di pubblicazione: 2018-05-02
Rated 5 di
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chip123 da
Good
All lines are clear and antibody is good,,,,,,,,,,,
Data di pubblicazione: 2018-04-06