Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Aquaporin 5/AQP5 CRISPR/Cas9 KO Plasmid (h): sc-400567
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Aquaporin 5/AQP5 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Aquaporin 5/AQP5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Aquaporin 5/AQP5 Antibody (D-7): sc-514022
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Aquaporin 5/AQP5 CRISPR/Cas9 KO Plasmid (h) | sc-400567 | 20 µg | $397.00 |
Overview
AQP5 encodes aquaporin 5, a membrane water channel that facilitates rapid, selective water transport across the plasma membrane in epithelial tissues. In secretory epithelia of the airway, salivary and lacrimal glands, and lung alveoli, AQP5 supports fluid homeostasis and contributes to mucosal hydration and barrier physiology by regulating transepithelial water movement. Its activity is integrated with osmotic stress responses, epithelial ion transport, and junctional organization that collectively shape airway surface liquid and glandular secretion. Dysregulated AQP5 expression or localization has been associated with epithelial dysfunction in inflammatory airway conditions, salivary gland hypofunction, and altered water handling in pulmonary pathophysiology, making it relevant for mechanistic studies of secretion and epithelial remodeling.
Aquaporin 5/AQP5 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the AQP5 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the AQP5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the AQP5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Aquaporin 5/AQP5 protein expression.
This CRISPR knockout system enables efficient generation of AQP5-deficient cell models for investigation of Aquaporin 5/AQP5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting AQP5 exon(s) critical for Aquaporin 5/AQP5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple AQP5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Aquaporin 5/AQP5 CRISPR/Cas9 KO Plasmid (h) and Aquaporin 5/AQP5 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the AQP5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Aquaporin 5/AQP5 HDR Plasmid (h) and Aquaporin 5/AQP5 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by AQP5 homology arms to support homology-directed repair at defined AQP5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.