AP-2α Antibody (3B5): sc-12726

AP-2α Antibody (3B5) is a mouse monoclonal IgG2b kappa light chain antibody that detects AP-2 alpha protein of mouse, rat, and human origin through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry (IHC). AP-2α monoclonal antibody (3B5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. AP-2 alpha protein is a crucial transcription factor that binds to the DNA consensus sequence CCCCAGGC, initiating the transcription of specific genes. This function is vital for regulating gene expression related to cell growth and differentiation, particularly in ectodermal-derived tissues. AP-2α (3B5) antibody is involved in the regulation of estrogen receptor expression and plays a significant role in the development of various tissues, with mutations in the AP-2α gene linked to serious developmental disorders such as microphthalmia and corneal clouding. AP-2α interacts with other members of the AP-2 transcription factor family, including AP-2β and AP-2γ, which together form the OB2-1 transcription factor complex that upregulates the proto-oncogene c-ErbB-2, often overexpressed in breast cancers. AP-2α′s ability to modulate gene expression highlights its importance in both normal development and disease processes, making AP-2α (3B5) antibody a valuable tool for researchers studying these critical biological pathways.

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AP-2α Antibody (3B5) References:

1. An interstitial deletion of 6p24-p25 proximal to the FKHL7 locus and including AP-2alpha that affects anterior eye chamber development.  |  Davies, AF., et al. 1999. J Med Genet. 36: 708-10. PMID: 10507730
2. Functional analysis of AP-2 alpha and mu2 subunits.  |  Motley, AM., et al. 2006. Mol Biol Cell. 17: 5298-308. PMID: 17035630
3. AP-2α inhibits hepatocellular carcinoma cell growth and migration.  |  Huang, W., et al. 2016. Int J Oncol. 48: 1125-34. PMID: 26780928
4. Cloning and expression of AP-2, a cell-type-specific transcription factor that activates inducible enhancer elements.  |  Williams, T., et al. 1988. Genes Dev. 2: 1557-69. PMID: 3063603
5. A positive feedback loop between Periostin and TGFβ1 induces and maintains the stemness of hepatocellular carcinoma cells via AP-2α activation.  |  Chen, G., et al. 2021. J Exp Clin Cancer Res. 40: 218. PMID: 34193219
6. AP-2α/AP-2β Transcription Factors Are Key Regulators of Epidermal Homeostasis.  |  Zhang, H., et al. 2024. J Invest Dermatol. 144: 1505-1521.e12. PMID: 38237728
7. Cloning and characterization of a second AP-2 transcription factor: AP-2 beta.  |  Moser, M., et al. 1995. Development. 121: 2779-88. PMID: 7555706
8. An alternatively spliced mRNA from the AP-2 gene encodes a negative regulator of transcriptional activation by AP-2.  |  Buettner, R., et al. 1993. Mol Cell Biol. 13: 4174-85. PMID: 8321221
9. Chromosomal mapping of the human and mouse homologues of two new members of the AP-2 family of transcription factors.  |  Williamson, JA., et al. 1996. Genomics. 35: 262-4. PMID: 8661133
10. A family of AP-2 proteins regulates c-erbB-2 expression in mammary carcinoma.  |  Bosher, JM., et al. 1996. Oncogene. 13: 1701-7. PMID: 8895516
11. Comparative analysis of AP-2 alpha and AP-2 beta gene expression during murine embryogenesis.  |  Moser, M., et al. 1997. Dev Dyn. 208: 115-24. PMID: 8989526
12. Identification of ERF-1 as a member of the AP2 transcription factor family.  |  McPherson, LA., et al. 1997. Proc Natl Acad Sci U S A. 94: 4342-7. PMID: 9113991

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-AP-2α Antibody (3B5) has 100 citations in a variety of scientific publications.

Primary antibodies like Anti-AP-2α Antibody (3B5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

AP-2α Antibody (3B5) Specifications

Alternate Product Name: TFAP2A

Product Clone Name: 3B5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids:

Epitope Target: AP-2α

Epitope Species: human

Product Reactivity/Detection: AP-2α

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 100 citations in various scientific publications

Available Conjugates/Forms for AP-2α Antibody (3B5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent