ACTL7B Antibody G-2 è un monoclonale di topo mouse IgG2a κ fornito in 200 µg/ml
generato contro gli amminoacidi 329-368 mappati vicino al C-terminus di ACTL7B di origine human
raccomandato per il rilevamento di ACTL7B di origine mouse, rat e human in WB, IP, IF e ELISA
m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for ACTL7B Antibody (G-2) for WB applications. These reagents are now offered in bundles with ACTL7B Antibody (G-2) (see ordering information below).
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possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
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Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
ACTL7B (actin-like 7B) is a member of the ARP family of actin-related proteins that contain an actin fold and are involved in spindle orientation, nuclear migration and chromatin remodeling events. Localized to the cytoplasm and expressed in the testis and prostate, ACTL7B is 415 amino acids in length and is encoded by a gene that is oriented in a head-to-head formation with the familial dysautonomia (FD) candidate region on chromosome 9. Although located in a region associated with FD, ACTL7B is not involved in the pathogenesis of the genetic disease. ACTL7B shares high sequence similarity with ACTL7A (actin-like 7A) and contains one conserved protein kinase C (PKC) site and one conserved cAMP/cGMP-dependent phosphorylation site. The human and mouse proteins share 88% sequence similarity.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
How much sample do you recommend loading on the gel for Western blot detection using ACTL7B (G-2): sc-514196?
Richiesta da: jerojero
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference:
Rated 5 di
Produced positive Western blot data of ACTL7BProduced positive Western blot data of ACTL7B expression in non-transfected and human ACTL7B transfected 293T whole cell lysates. -SCBT QC
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