Anticorps Vitamin D Receptor/VDR (D-6) recommandé pour la détection de VDR d'origine mouse, rat et human par WB, IP, IF et IHC(P)

Anticorps Vitamin D Receptor/VDR (D-6): sc-13133

Anticorps Vitamin D Receptor/VDR (D-6) est évalué 4.6 de 5 de 30.
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Fiches techniques

    • Anti-L'anticorps Vitamin D Receptor/VDR D-6 est un monoclonal IgG2a κ L'Anticorps Vitamin D Receptor/VDR, cité dans 344 publications, fourni en 200 µg/ml
    • dirigé contre les acides aminés 344-424 de VDR d'origine human
    • Anti-L'Anticorps Vitamin D Receptor/VDR (D-6) est recommandé pour la détection de VDR d'origine mouse, rat et human par WB, IP, IF et IHC(P)
    • Anti-L'Anticorps Vitamin D Receptor/VDR (D-6) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
    • également disponible conjugué à la biotine pour WB, IHC(P) et ELISA
    • Réactif TransCruz pour une utilisation en Gel Supershift et ChIP (sc-13133 X, 200 µg/0.1 ml)
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de Vitamin D Receptor/VDR (D-6): sc-13133.
    • m-IgG Fc BP-HRP (protéine de liaison IgG Fc-HRP de souris) est le réactif secondaire recommandé pour la détection de l'anticorps Vitamin D Receptor/VDR (D-6) pour des applications de WB and IHC(P). Ce réactif est maintenant proposé en kit avec l'anticorps Vitamin D Receptor/VDR (D-6) (voir Informations pour la commande ci-dessous).

Hi there, since the VDR has different isoforms, I am wondering the amino acids sequence for VDR this antibody corresponding. Thanks!

Posée par: Fay Liu
Thank you for your question. VDR Antibody (D-6): sc-13133 was raised against amino acids 344-424 of VDR isoform 1 of human origin. This is the corresponding amino acid sequence: PGVQDAA LIEAIQDRLS NTLQTYIRCR HPPPGSHLLY AKMIQKLADL RSLNEEHSKQ YRCLSFQPEC SMKLTPLVLE VFGN Please do not hesitate to contact our technical service directly if you have additional questions.
Répondue par: Tech Support Europe
Date de publication: 2019-06-05

does D6 vitamin d receptor antibody cross react with monkey (macaques) for immunohistochemistry

Posée par: schristak
Thank you for your question. This antibody has not been tested on monkey samples in house, so it is not technically recommended or warrantied for that use, however, we do have publications in monkey (rhesus) samples.
Répondue par: Tech Service
Date de publication: 2019-04-09

Hello, I have been working with another VDR and I am not certain I am fully appreciating that VDR. Hence I am interested in the "Contact us to receive a FREE 10 µg sample of VDR (D-6): sc-13133." offer of getting 10 ug for free. Is it possible?

Posée par: hanna abrahamsson
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Répondue par: SCBT Heidelberg Support
Date de publication: 2018-12-14

Can Santa Cruz provide VDR IHC images (performed using D-6 sc-13133 ab) with staining intensity (score) of 1 to 4 and % positive stained cells (score) with 1-4 (preferably in FFPE colorectal tumor tissue samples) ?

Posée par: Sanbc
Thank you for the question. Please note that this product is sold for research or laboratory use only and it must not been used for medical diagnostics. All available IHC(P) images for this product will be published upon availability on our product website. We currently show staining of human parathyroid gland tissue showing nuclear and cytoplasmic staining of glandular cells, human skin tissue showing nuclear and cytoplasmic staining of keratinocytes, fibroblasts, Langerhans cells and melanocytes and human adrenal gland tissue showing nuclear staining of cortical cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Répondue par: Technical Support Europe
Date de publication: 2018-01-10

I got a double band when I used this antibody, any idea what the other band could be? Thanks

Posée par: Reham
Thank you very much for your inquiry. Please understand that we do not interpret customer data, since we do not have a detailed knowledge about your experiment and how the data was produced. Therefore our interpretation of the data could lead to a wrong direction without knowing all the relevant details. However, Western blotting is a technique that separates proteins based on size. In general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors, so the actual band size observed may differ from that predicted. Common factors include: 1. Post-translational modification - e.g. phosphorylation, glycosylation etc, which increases the size of the protein. 2. Post-translation cleavage - e.g. many proteins are synthesized as pro-proteins and then cleaved to give the active form, e.g. pro-caspases. 3. Splice variants - alternative splicing may create different sized proteins produced from the same gene. 4. Relative charge - the composition of amino acids (charged vs non-charged). 5. Multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.
Répondue par: Technical Support Europe
Date de publication: 2018-09-01

What is VDR D-6 best diluted in?

Posée par: Daraaaa
Thank you for your question. What it is best diluted in depends on the application you plan on using it for. For Western blot, we would recommend diluting it in 5% milk with TBS-T. For IF/IHC, we would recommend diluting it in blocking serum. You can find more information in the protocol you plan on following: https://www.scbt.com/scbt/resources/protocols
Répondue par: Tech Service
Date de publication: 2017-05-25

Hello, Does this ab work fine when it is conjugated to a regular anti-mouse IgG? Thanks!

Posée par: MMac
Thank you for your question. Yes, this antibody can be used with a conventional anti-mouse IgG secondary or our Mouse IgG Binding Protein, both are appropriate.
Répondue par: Technical Service
Date de publication: 2017-04-24

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Posée par: Cweed
Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. SC-13133 is available directly conjugated to FITC, AF488, and AF647.
Répondue par: TechService7
Date de publication: 2021-01-21
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Rated 5 de 5 de par Worked well in human and mouse muscle tissue. I used this antibody for IHC and got good results following published protocols. I used the unconjugated primary antibody and had to amplify for the human samples, but the mouse samples worked well going straight into secodary.
Date de publication: 2021-01-25
Rated 3 de 5 de par low dilution I needed to use a low dilution to make it work (1:50)
Date de publication: 2020-09-22
Rated 5 de 5 de par Buen anticuerpo Tiene una alta especificidad, en wblot da una muy buena señal y se puede reutilizar varias veces.
Date de publication: 2020-09-01
Rated 5 de 5 de par very good antibody This antibody works very well in Wb. Also worked in IF in a PLA assay. Very satisfied.
Date de publication: 2019-01-02
Rated 5 de 5 de par Best Antibody We have tried this antibody and published. Instruction and protocol was well described. I strongly recommend this antibody to other researcher.
Date de publication: 2018-08-24
Rated 5 de 5 de par EXCELLENT SENSITIVITY TO SEE VDR ON PROTEIN LEVEL IS VERY TOUGH, BUT USING THE HYPEROSMOLAR BUFFER LYSIS AND THIS VDR ANTIBODY. I GOT THE BAND OF VDR. THIS ANTIBODY IS BETTER THAN ABCAM. THANK YOU
Date de publication: 2018-04-12
Rated 5 de 5 de par Works well for immunofluorescence and wester blot This antibody works very well at 1:1000 dilution for western blot and at 1:200 dilution for immunofluorescence on mouse calvariae.
Date de publication: 2018-03-16
Rated 4 de 5 de par Arterial immunohistochemistry VDR antibody is a nuclear antibody, but the cytoplasm is also stained. Look at the pictures provided by manual also have cytoplasmic staining. Do you know how to explain properly?
Date de publication: 2017-10-16
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