Tris hydrochloride solution, pH 7.4 CAS: 1185-53-1 (solid)
MF: NH2C(CH2OH)3·HCl
MW: 157.60
Non-sterile; 0.2 µm filtered, 1M stock solution used to make buffers.

Tris hydrochloride solution, pH 7.4 (CAS 1185-53-1 (solid))

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Noms alternatifs: Tris(hydroxymethyl)aminomethane hydrochloride solution, pH 7.4
Application: Tris hydrochloride solution, pH 7.4 is non-sterile; 0.2 µm filtered, 1M stock solution used to make buffers
Numéro CAS: 1185-53-1 (solid)
Masse Moléculaire: 157.60
Formule Moléculaire: NH2C(CH2OH)3·HCl
Pour la Recherche Uniquement. Non conforme pour le Diagnostic ou pour une Utilisation Thérapeutique.
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pH 7.4, 1 M. Tris hydrochloride has been used as a component of extraction buffer e.g. phenol extraction of DNA or RNA; and as a component of separating and stacking gels in the characterization of in vitro translation products by SDS-PAGE. The pH values of Tris buffer solutions are temperature and concentration-dependent. Between 5 °C and 25 °C, the pH value increases an average of 0.03 pH units for each °C decrease in temperature. As the buffer temperature increases from 25 °C to 37 °C, the pH value decreases an average of 0.025 pH units per °C. Increasing the concentration of Tris from 0.05 M to 0.5M will increase the pH value by about 0.05 units. Decreasing the concentration from 0.05 M to 0.005 M will decrease the pH value by about 0.05 units.

Formulation :
1 M
État Physique :
Liquid
pH :
7.4
STOCKAGE :
Store at room temperature
Pour la Recherche Uniquement. Non conforme pour le Diagnostic ou pour une Utilisation Thérapeutique.
WGK Allemagne :
2
PubChem CID :
SMILES :
C(C(CO)(CO)N)O.Cl

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