Towbin, without SDS is a western blot transfer buffer for use with nitrocellulose and PVDF transfer membranes. Towbin buffer is a Tris-glycine buffer with a pH of 8.3.This product is recommended for use when transferring proteins that are highly soluble and have proven to elute from gels easily. If protein transfer is inefficient, a suitable alternative may be: Towbin, with SDS, 10X (sc-24954).
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2. Towbin, H., et al. 1992. Biotechnology. 24: 145-149. PMID: 1422008